miRNA: 3'- -CUAAUUGGGAAaUUuAGGUACAACAA- -5'
miRNA: 3'- cuAAUUGGGAAAUUuAGGUACAACAA- -5'
miRNA: 3'- -CUAAUUGGGAAAUUUAGGU---ACAACaa -5'
miRNA: 3'- -CUAAUUGGGAAAUUUAGGUACAACAA- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28274 | 3' | -40.2 | NC_005902.1 | + | 140891 | 0.81 | 0.987375 |
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Target: 5'- gGGUUAAUCCUUaAAcUCCAUGUUGUUg -3' miRNA: 3'- -CUAAUUGGGAAaUUuAGGUACAACAA- -5' |
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| 28274 | 3' | -40.2 | NC_005902.1 | + | 23353 | 0.87 | 0.880063 |
|
Target: 5'- -uUUAACCCUUUAAuUCUAUGUUGUUa -3' miRNA: 3'- cuAAUUGGGAAAUUuAGGUACAACAA- -5' |
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| 28274 | 3' | -40.2 | NC_005902.1 | + | 11175 | 0.9 | 0.744714 |
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Target: 5'- gGGUUAACCCUUUAAAUCCAcauUGUUGa- -3' miRNA: 3'- -CUAAUUGGGAAAUUUAGGU---ACAACaa -5' |
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| 28274 | 3' | -40.2 | NC_005902.1 | + | 11121 | 1.08 | 0.136135 |
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Target: 5'- gGAUUAACCCUUUAAAUCCAUGUUGUUg -3' miRNA: 3'- -CUAAUUGGGAAAUUUAGGUACAACAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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