Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28285 | 3' | -44.5 | NC_005902.1 | + | 90794 | 0.66 | 0.999999 |
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Target: 5'- --cCCUUUAAAUCCUaaGGC--UUAUACa -3' miRNA: 3'- guaGGAAAUUUGGGA--CCGcuAAUAUG- -5' |
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| 28285 | 3' | -44.5 | NC_005902.1 | + | 81993 | 0.66 | 0.999999 |
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Target: 5'- uUAUCCUUUAAAUCCUaaGGUG-UUAUu- -3' miRNA: 3'- -GUAGGAAAUUUGGGA--CCGCuAAUAug -5' |
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| 28285 | 3' | -44.5 | NC_005902.1 | + | 81352 | 0.66 | 0.999999 |
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Target: 5'- uUAUCCUUUAAAUCCUaagGGUUGUAUu -3' miRNA: 3'- -GUAGGAAAUUUGGGAccgCUAAUAUG- -5' |
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| 28285 | 3' | -44.5 | NC_005902.1 | + | 163699 | 0.69 | 0.999939 |
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Target: 5'- aAUCCUUUAAAUCCUaaGGC---UAUACu -3' miRNA: 3'- gUAGGAAAUUUGGGA--CCGcuaAUAUG- -5' |
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| 28285 | 3' | -44.5 | NC_005902.1 | + | 168611 | 0.71 | 0.999517 |
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Target: 5'- --cCCUUUAAACCCUaaGGC--UUAUACu -3' miRNA: 3'- guaGGAAAUUUGGGA--CCGcuAAUAUG- -5' |
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| 28285 | 3' | -44.5 | NC_005902.1 | + | 100274 | 0.72 | 0.997939 |
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Target: 5'- uUAUCCUUUAAAUCCUaaGGC--UUAUACu -3' miRNA: 3'- -GUAGGAAAUUUGGGA--CCGcuAAUAUG- -5' |
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| 28285 | 3' | -44.5 | NC_005902.1 | + | 139740 | 0.72 | 0.997939 |
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Target: 5'- uUAUCCUUUAAAUCCUaaGGC--UUAUACu -3' miRNA: 3'- -GUAGGAAAUUUGGGA--CCGcuAAUAUG- -5' |
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| 28285 | 3' | -44.5 | NC_005902.1 | + | 184514 | 0.72 | 0.997939 |
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Target: 5'- gUAUCCUUUAAAUCCUaaGGC--UUAUACu -3' miRNA: 3'- -GUAGGAAAUUUGGGA--CCGcuAAUAUG- -5' |
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| 28285 | 3' | -44.5 | NC_005902.1 | + | 168357 | 0.77 | 0.95477 |
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Target: 5'- uCAUCCUUUAAACCCUaaGGC--UUAUAUa -3' miRNA: 3'- -GUAGGAAAUUUGGGA--CCGcuAAUAUG- -5' |
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| 28285 | 3' | -44.5 | NC_005902.1 | + | 81094 | 1.13 | 0.026338 |
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Target: 5'- aCAUCCUUUAAACCCUGGCGAUUAUACa -3' miRNA: 3'- -GUAGGAAAUUUGGGACCGCUAAUAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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