miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
28350 5' -54.2 NC_005946.1 + 50956 0.66 0.869019
Target:  5'- cUCC-CGCCCCCUcuacguCUGGGuUUACAa -3'
miRNA:   3'- uAGGaGUGGGGGGu-----GACCUuAAUGUg -5'
28350 5' -54.2 NC_005946.1 + 76863 0.66 0.869019
Target:  5'- gAUCC-CGCCCauCCC-CUGGAcg-ACACg -3'
miRNA:   3'- -UAGGaGUGGG--GGGuGACCUuaaUGUG- -5'
28350 5' -54.2 NC_005946.1 + 82954 0.67 0.818275
Target:  5'- -cCCUCAgCCCCgGCgGGGAUggaaaACGCu -3'
miRNA:   3'- uaGGAGUgGGGGgUGaCCUUAa----UGUG- -5'
28350 5' -54.2 NC_005946.1 + 1868 0.67 0.817367
Target:  5'- cUCCUCccacuCCCCCCACUccccccacucccaGGccggUGCACa -3'
miRNA:   3'- uAGGAGu----GGGGGGUGA-------------CCuua-AUGUG- -5'
28350 5' -54.2 NC_005946.1 + 11038 0.71 0.634611
Target:  5'- uGUCCUCACCCUCUcccagucuGCcagGGggUgGCACa -3'
miRNA:   3'- -UAGGAGUGGGGGG--------UGa--CCuuAaUGUG- -5'
28350 5' -54.2 NC_005946.1 + 13270 1.07 0.003334
Target:  5'- cAUCCUCACCCCCCACUGGAAUUACACc -3'
miRNA:   3'- -UAGGAGUGGGGGGUGACCUUAAUGUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.