Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28353 | 5' | -57.3 | NC_005946.1 | + | 71316 | 0.73 | 0.367958 |
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Target: 5'- uGGGAcagcguCCUCCacUCGGGCUCGACGGGCa -3' miRNA: 3'- cCUCU------GGAGG--AGUUCGGGCUGCUCGa -5' |
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| 28353 | 5' | -57.3 | NC_005946.1 | + | 98076 | 0.73 | 0.38453 |
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Target: 5'- gGGAGACCUCCcgCAaggucggaGGCCaCGAUGGGgCUg -3' miRNA: 3'- -CCUCUGGAGGa-GU--------UCGG-GCUGCUC-GA- -5' |
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| 28353 | 5' | -57.3 | NC_005946.1 | + | 104021 | 0.72 | 0.41912 |
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Target: 5'- cGGAGGCCaCCUUAcccgccAGCUgGugGAGCUg -3' miRNA: 3'- -CCUCUGGaGGAGU------UCGGgCugCUCGA- -5' |
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| 28353 | 5' | -57.3 | NC_005946.1 | + | 102164 | 0.7 | 0.55317 |
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Target: 5'- aGGGACCUCCUCAugAGCUCGuagucgucCGAGgUg -3' miRNA: 3'- cCUCUGGAGGAGU--UCGGGCu-------GCUCgA- -5' |
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| 28353 | 5' | -57.3 | NC_005946.1 | + | 27830 | 0.7 | 0.563328 |
|
Target: 5'- cGGGGGcCCUCCUCGacAGCuuGuccuCGGGCc -3' miRNA: 3'- -CCUCU-GGAGGAGU--UCGggCu---GCUCGa -5' |
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| 28353 | 5' | -57.3 | NC_005946.1 | + | 34758 | 0.69 | 0.62507 |
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Target: 5'- gGGGGACUUUCUCccaaacugccaGGGCUCGAUGAuGCg -3' miRNA: 3'- -CCUCUGGAGGAG-----------UUCGGGCUGCU-CGa -5' |
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| 28353 | 5' | -57.3 | NC_005946.1 | + | 38750 | 0.66 | 0.80372 |
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Target: 5'- cGGAGACCUCCUgGgugaAGCCacuCuGGCUu -3' miRNA: 3'- -CCUCUGGAGGAgU----UCGGgcuGcUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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