Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28353 | 5' | -57.3 | NC_005946.1 | + | 71316 | 0.73 | 0.367958 |
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Target: 5'- uGGGAcagcguCCUCCacUCGGGCUCGACGGGCa -3' miRNA: 3'- cCUCU------GGAGG--AGUUCGGGCUGCUCGa -5' |
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| 28353 | 5' | -57.3 | NC_005946.1 | + | 95444 | 0.74 | 0.344035 |
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Target: 5'- gGGAGACCcgCCgCAGGCUCGA-GAGCa -3' miRNA: 3'- -CCUCUGGa-GGaGUUCGGGCUgCUCGa -5' |
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| 28353 | 5' | -57.3 | NC_005946.1 | + | 61448 | 0.77 | 0.22995 |
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Target: 5'- -aGGAUgCUCCUCAGGCCCGACGuaaaguGGCUg -3' miRNA: 3'- ccUCUG-GAGGAGUUCGGGCUGC------UCGA- -5' |
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| 28353 | 5' | -57.3 | NC_005946.1 | + | 38698 | 0.78 | 0.202567 |
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Target: 5'- cGGAuGGCCUCCUCGAGCUCGGCcuuccucaugguaGAGUa -3' miRNA: 3'- -CCU-CUGGAGGAGUUCGGGCUG-------------CUCGa -5' |
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| 28353 | 5' | -57.3 | NC_005946.1 | + | 49278 | 0.8 | 0.149499 |
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Target: 5'- --cGACCUCCUguAGCCCGACGGGaCg -3' miRNA: 3'- ccuCUGGAGGAguUCGGGCUGCUC-Ga -5' |
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| 28353 | 5' | -57.3 | NC_005946.1 | + | 88319 | 0.81 | 0.121273 |
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Target: 5'- -uGGACCUCCUCAGGCgCGAgGGGCa -3' miRNA: 3'- ccUCUGGAGGAGUUCGgGCUgCUCGa -5' |
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| 28353 | 5' | -57.3 | NC_005946.1 | + | 13921 | 1.1 | 0.001314 |
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Target: 5'- gGGAGACCUCCUCAAGCCCGACGAGCUg -3' miRNA: 3'- -CCUCUGGAGGAGUUCGGGCUGCUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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