Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28356 | 3' | -57 | NC_005946.1 | + | 82685 | 0.69 | 0.638334 |
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Target: 5'- aGCUCaCGUCGgagggcccuACGCGGgugcaaugGCUC-CCUCCCa -3' miRNA: 3'- -CGGG-GCAGU---------UGCGUC--------UGAGaGGAGGG- -5' |
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| 28356 | 3' | -57 | NC_005946.1 | + | 83191 | 0.68 | 0.68961 |
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Target: 5'- gGCCCCG-CAcAgGCAGucCUCUUCgCCCa -3' miRNA: 3'- -CGGGGCaGU-UgCGUCu-GAGAGGaGGG- -5' |
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| 28356 | 3' | -57 | NC_005946.1 | + | 64993 | 0.68 | 0.709844 |
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Target: 5'- gGCgUCGUCAGCcuCAGcCUCUCUaUCCCg -3' miRNA: 3'- -CGgGGCAGUUGc-GUCuGAGAGG-AGGG- -5' |
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| 28356 | 3' | -57 | NC_005946.1 | + | 63610 | 0.68 | 0.709844 |
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Target: 5'- aCCUCGUa---GCAGAgcaucCUCUCCUCCa -3' miRNA: 3'- cGGGGCAguugCGUCU-----GAGAGGAGGg -5' |
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| 28356 | 3' | -57 | NC_005946.1 | + | 38339 | 0.68 | 0.709844 |
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Target: 5'- cCCCCGUCAuguUGUGGAUUCcuaUCCCg -3' miRNA: 3'- cGGGGCAGUu--GCGUCUGAGaggAGGG- -5' |
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| 28356 | 3' | -57 | NC_005946.1 | + | 20960 | 0.68 | 0.709844 |
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Target: 5'- aGUCauaCGUgGugGCGGAC-CUggCCUCCCa -3' miRNA: 3'- -CGGg--GCAgUugCGUCUGaGA--GGAGGG- -5' |
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| 28356 | 3' | -57 | NC_005946.1 | + | 93991 | 0.66 | 0.831505 |
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Target: 5'- cGCCCCGgc--CGUccuGACUgaCCUCCUg -3' miRNA: 3'- -CGGGGCaguuGCGu--CUGAgaGGAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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