Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28356 | 3' | -57 | NC_005946.1 | + | 72670 | 0.67 | 0.784579 |
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Target: 5'- aCCCCGUgGgguucgugagggagGC-CAGGCUCUgUCUCCCc -3' miRNA: 3'- cGGGGCAgU--------------UGcGUCUGAGA-GGAGGG- -5' |
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| 28356 | 3' | -57 | NC_005946.1 | + | 99353 | 0.66 | 0.805499 |
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Target: 5'- uGCUCggUGUCugaGCAGACgaugcgCUCCUCCg -3' miRNA: 3'- -CGGG--GCAGuugCGUCUGa-----GAGGAGGg -5' |
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| 28356 | 3' | -57 | NC_005946.1 | + | 18787 | 0.66 | 0.805499 |
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Target: 5'- aCCCCGUCuuuGCuCAGACcCUCUUCg- -3' miRNA: 3'- cGGGGCAGu--UGcGUCUGaGAGGAGgg -5' |
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| 28356 | 3' | -57 | NC_005946.1 | + | 15018 | 0.66 | 0.805499 |
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Target: 5'- cCCCCGUCA----AGACUCcUCCgaCCCa -3' miRNA: 3'- cGGGGCAGUugcgUCUGAG-AGGa-GGG- -5' |
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| 28356 | 3' | -57 | NC_005946.1 | + | 34135 | 0.66 | 0.814338 |
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Target: 5'- -aCCUGUCGACggGCAuGACgUCcgUCCUCCUg -3' miRNA: 3'- cgGGGCAGUUG--CGU-CUG-AG--AGGAGGG- -5' |
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| 28356 | 3' | -57 | NC_005946.1 | + | 100961 | 0.66 | 0.823009 |
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Target: 5'- gGCaCCCGUCG----AGACUCUUgUCCUg -3' miRNA: 3'- -CG-GGGCAGUugcgUCUGAGAGgAGGG- -5' |
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| 28356 | 3' | -57 | NC_005946.1 | + | 93991 | 0.66 | 0.831505 |
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Target: 5'- cGCCCCGgc--CGUccuGACUgaCCUCCUg -3' miRNA: 3'- -CGGGGCaguuGCGu--CUGAgaGGAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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