Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28356 | 3' | -57 | NC_005946.1 | + | 82685 | 0.69 | 0.638334 |
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Target: 5'- aGCUCaCGUCGgagggcccuACGCGGgugcaaugGCUC-CCUCCCa -3' miRNA: 3'- -CGGG-GCAGU---------UGCGUC--------UGAGaGGAGGG- -5' |
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| 28356 | 3' | -57 | NC_005946.1 | + | 66460 | 0.7 | 0.576712 |
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Target: 5'- cGCCU--UCAACGCcgugcgAGAgUCUUCUCCCa -3' miRNA: 3'- -CGGGgcAGUUGCG------UCUgAGAGGAGGG- -5' |
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| 28356 | 3' | -57 | NC_005946.1 | + | 68071 | 0.71 | 0.556415 |
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Target: 5'- uUCCCGUCAG-GCAGAgCUCcUCUUCCa -3' miRNA: 3'- cGGGGCAGUUgCGUCU-GAG-AGGAGGg -5' |
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| 28356 | 3' | -57 | NC_005946.1 | + | 72467 | 0.71 | 0.546343 |
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Target: 5'- uCCUCGUCccCGuCGGACUCUC-UCCCu -3' miRNA: 3'- cGGGGCAGuuGC-GUCUGAGAGgAGGG- -5' |
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| 28356 | 3' | -57 | NC_005946.1 | + | 30458 | 0.71 | 0.516503 |
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Target: 5'- aGCCCgGUCGcUGC--GCUCUCCacUCCCa -3' miRNA: 3'- -CGGGgCAGUuGCGucUGAGAGG--AGGG- -5' |
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| 28356 | 3' | -57 | NC_005946.1 | + | 8123 | 0.75 | 0.347412 |
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Target: 5'- aCCCUGUCcACGuCAGAgUC-CCUCCCc -3' miRNA: 3'- cGGGGCAGuUGC-GUCUgAGaGGAGGG- -5' |
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| 28356 | 3' | -57 | NC_005946.1 | + | 16608 | 1.12 | 0.001034 |
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Target: 5'- gGCCCCGUCAACGCAGACUCUCCUCCCc -3' miRNA: 3'- -CGGGGCAGUUGCGUCUGAGAGGAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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