Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28369 | 3' | -53.3 | NC_005946.1 | + | 98189 | 0.66 | 0.951465 |
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Target: 5'- --gGGCGGCCUGCaCCUg-----CUCCa -3' miRNA: 3'- gaaCUGCUGGACG-GGAaagccaGAGG- -5' |
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| 28369 | 3' | -53.3 | NC_005946.1 | + | 32509 | 0.66 | 0.951465 |
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Target: 5'- uCUUGACGuCCuUGUCCUUgaGGUgCUUCu -3' miRNA: 3'- -GAACUGCuGG-ACGGGAAagCCA-GAGG- -5' |
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| 28369 | 3' | -53.3 | NC_005946.1 | + | 47781 | 0.66 | 0.947113 |
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Target: 5'- uCUUGugGGCCcuguugaGCUCg-UCGGUCUgCa -3' miRNA: 3'- -GAACugCUGGa------CGGGaaAGCCAGAgG- -5' |
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| 28369 | 3' | -53.3 | NC_005946.1 | + | 22512 | 0.66 | 0.937657 |
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Target: 5'- --cGGCGcacucuGCCUcgaggGCCCUguaagUCGGUcCUCCu -3' miRNA: 3'- gaaCUGC------UGGA-----CGGGAa----AGCCA-GAGG- -5' |
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| 28369 | 3' | -53.3 | NC_005946.1 | + | 86291 | 0.66 | 0.926633 |
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Target: 5'- aCUUGACGACCaugGUCUUuauaacgUUUGGcuuaUCUCCc -3' miRNA: 3'- -GAACUGCUGGa--CGGGA-------AAGCC----AGAGG- -5' |
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| 28369 | 3' | -53.3 | NC_005946.1 | + | 40271 | 0.7 | 0.802973 |
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Target: 5'- -cUGACGGCCUcuccgGCaaaCUgcuuauaguaguugUCGGUCUCCu -3' miRNA: 3'- gaACUGCUGGA-----CGg--GAa-------------AGCCAGAGG- -5' |
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| 28369 | 3' | -53.3 | NC_005946.1 | + | 78959 | 0.74 | 0.552741 |
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Target: 5'- gCUUGGCGuACCUGCUCagUauGUCUCCc -3' miRNA: 3'- -GAACUGC-UGGACGGGaaAgcCAGAGG- -5' |
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| 28369 | 3' | -53.3 | NC_005946.1 | + | 89844 | 0.76 | 0.462561 |
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Target: 5'- --cGAUGACCgUGCCCccgUCaGUCUCCa -3' miRNA: 3'- gaaCUGCUGG-ACGGGaa-AGcCAGAGG- -5' |
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| 28369 | 3' | -53.3 | NC_005946.1 | + | 28912 | 1.13 | 0.002196 |
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Target: 5'- aCUUGACGACCUGCCCUUUCGGUCUCCu -3' miRNA: 3'- -GAACUGCUGGACGGGAAAGCCAGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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