Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28552 | 5' | -57.5 | NC_005946.1 | + | 2284 | 0.67 | 0.700831 |
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Target: 5'- cGGACCccgCcGGGG-GCUGGUGCAGa- -3' miRNA: 3'- cCCUGGa--GaCUCCaCGACCGCGUUcc -5' |
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| 28552 | 5' | -57.5 | NC_005946.1 | + | 13348 | 0.67 | 0.696767 |
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Target: 5'- cGGGGCCUacauaucccugcugCUGggguccaGGGUGgUGGC-CGAGGg -3' miRNA: 3'- -CCCUGGA--------------GAC-------UCCACgACCGcGUUCC- -5' |
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| 28552 | 5' | -57.5 | NC_005946.1 | + | 56450 | 0.68 | 0.68043 |
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Target: 5'- uGGACCUCUGgagucgaugGGGUGCUcaugGGCuCcAGGa -3' miRNA: 3'- cCCUGGAGAC---------UCCACGA----CCGcGuUCC- -5' |
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| 28552 | 5' | -57.5 | NC_005946.1 | + | 12097 | 0.68 | 0.663989 |
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Target: 5'- aGGGACuuaugccccacgcgaCUCUGAGGgagaugagggaGCUGGCGgAgcAGGa -3' miRNA: 3'- -CCCUG---------------GAGACUCCa----------CGACCGCgU--UCC- -5' |
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| 28552 | 5' | -57.5 | NC_005946.1 | + | 67932 | 0.68 | 0.658836 |
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Target: 5'- aGGGACCUCucguUGGGGUacuGCUgcacGGCGUAgccccucucggccAGGa -3' miRNA: 3'- -CCCUGGAG----ACUCCA---CGA----CCGCGU-------------UCC- -5' |
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| 28552 | 5' | -57.5 | NC_005946.1 | + | 94699 | 0.7 | 0.567158 |
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Target: 5'- gGGGGcCCUCaGAGGagGaUUGGCGuCGAGGa -3' miRNA: 3'- -CCCU-GGAGaCUCCa-C-GACCGC-GUUCC- -5' |
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| 28552 | 5' | -57.5 | NC_005946.1 | + | 48268 | 0.71 | 0.497329 |
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Target: 5'- aGGGugCUg-GGGGUGCUugcaGGCGUAGGu -3' miRNA: 3'- -CCCugGAgaCUCCACGA----CCGCGUUCc -5' |
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| 28552 | 5' | -57.5 | NC_005946.1 | + | 25787 | 0.71 | 0.478069 |
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Target: 5'- cGGACC-CgGAGGUGgUGGaCGaCGAGGa -3' miRNA: 3'- cCCUGGaGaCUCCACgACC-GC-GUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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