Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28564 | 5' | -62.1 | NC_005946.1 | + | 72200 | 0.66 | 0.577192 |
Target: 5'- uACCCCcaaaagAGCCUGGUGGACUCUagguccguaaaGAGGCu -3' miRNA: 3'- -UGGGG------UCGGACUGCCUGGGG-----------CUCUGc -5' |
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28564 | 5' | -62.1 | NC_005946.1 | + | 49439 | 0.66 | 0.577192 |
Target: 5'- aACCCCuccagGGCCcuggagggGACGGGguCCUCGuAGACGg -3' miRNA: 3'- -UGGGG-----UCGGa-------CUGCCU--GGGGC-UCUGC- -5' |
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28564 | 5' | -62.1 | NC_005946.1 | + | 36682 | 0.66 | 0.577192 |
Target: 5'- aGCCUCucuCCUGACGGGCCUgGgcucGGACu -3' miRNA: 3'- -UGGGGuc-GGACUGCCUGGGgC----UCUGc -5' |
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28564 | 5' | -62.1 | NC_005946.1 | + | 9073 | 0.66 | 0.547693 |
Target: 5'- -aCCUGGCCUGG-GGACCCugCGGGAgGu -3' miRNA: 3'- ugGGGUCGGACUgCCUGGG--GCUCUgC- -5' |
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28564 | 5' | -62.1 | NC_005946.1 | + | 27437 | 0.66 | 0.547693 |
Target: 5'- cGCCCuCGGCCgaGAUGaGGCuCCUGAGGuCGg -3' miRNA: 3'- -UGGG-GUCGGa-CUGC-CUG-GGGCUCU-GC- -5' |
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28564 | 5' | -62.1 | NC_005946.1 | + | 42360 | 0.66 | 0.537962 |
Target: 5'- gACCCUuugGGCCgu-CGGGCCCCGcGuCGu -3' miRNA: 3'- -UGGGG---UCGGacuGCCUGGGGCuCuGC- -5' |
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28564 | 5' | -62.1 | NC_005946.1 | + | 7752 | 0.66 | 0.537962 |
Target: 5'- gAUCCCAuCCUGgggucguaaACGGACCCCgucucugccuuGAGGCa -3' miRNA: 3'- -UGGGGUcGGAC---------UGCCUGGGG-----------CUCUGc -5' |
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28564 | 5' | -62.1 | NC_005946.1 | + | 95362 | 0.67 | 0.518686 |
Target: 5'- gGCCCaggGGCUUcGGgGGAggcagguuccCCCCGAGGCGa -3' miRNA: 3'- -UGGGg--UCGGA-CUgCCU----------GGGGCUCUGC- -5' |
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28564 | 5' | -62.1 | NC_005946.1 | + | 102049 | 0.67 | 0.50915 |
Target: 5'- gGCCCCuGGuCUUGACGaGCCUCucgGAGACGg -3' miRNA: 3'- -UGGGG-UC-GGACUGCcUGGGG---CUCUGC- -5' |
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28564 | 5' | -62.1 | NC_005946.1 | + | 74135 | 0.67 | 0.490307 |
Target: 5'- aGCCCCcguGCCcaGACGGcaaACCCUGGGAg- -3' miRNA: 3'- -UGGGGu--CGGa-CUGCC---UGGGGCUCUgc -5' |
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28564 | 5' | -62.1 | NC_005946.1 | + | 42300 | 0.68 | 0.462671 |
Target: 5'- gACCCCuggGGCCcuuugGACccucaGGACCCgCGGGACc -3' miRNA: 3'- -UGGGG---UCGGa----CUG-----CCUGGG-GCUCUGc -5' |
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28564 | 5' | -62.1 | NC_005946.1 | + | 42876 | 0.68 | 0.44471 |
Target: 5'- cAUCCCAGCCUGGacacCGGGa-UCGAGugGa -3' miRNA: 3'- -UGGGGUCGGACU----GCCUggGGCUCugC- -5' |
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28564 | 5' | -62.1 | NC_005946.1 | + | 26984 | 0.68 | 0.435876 |
Target: 5'- cACCCUcuuuAGCCUGugGG-CCuuGGuGGCGu -3' miRNA: 3'- -UGGGG----UCGGACugCCuGGggCU-CUGC- -5' |
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28564 | 5' | -62.1 | NC_005946.1 | + | 47606 | 0.68 | 0.434122 |
Target: 5'- cACCCUGGCCgcaaaguccuccGGCGGAacgugccuggaaCCCUGAGGCa -3' miRNA: 3'- -UGGGGUCGGa-----------CUGCCU------------GGGGCUCUGc -5' |
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28564 | 5' | -62.1 | NC_005946.1 | + | 12603 | 0.68 | 0.433246 |
Target: 5'- uGCCCCAGgagguggaggagcuCCUGACGGugUCUcccgugGAGACc -3' miRNA: 3'- -UGGGGUC--------------GGACUGCCugGGG------CUCUGc -5' |
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28564 | 5' | -62.1 | NC_005946.1 | + | 23316 | 0.68 | 0.418518 |
Target: 5'- cGCCCCGGCCUuACGGuCCUCaGGAa- -3' miRNA: 3'- -UGGGGUCGGAcUGCCuGGGGcUCUgc -5' |
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28564 | 5' | -62.1 | NC_005946.1 | + | 26766 | 0.69 | 0.385098 |
Target: 5'- uACCCCAGCUaGGgGGAgucucgacguCCCCGaAGGCa -3' miRNA: 3'- -UGGGGUCGGaCUgCCU----------GGGGC-UCUGc -5' |
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28564 | 5' | -62.1 | NC_005946.1 | + | 94806 | 0.7 | 0.361219 |
Target: 5'- aACCCCAGgCUGAauauaCCCGAGACu -3' miRNA: 3'- -UGGGGUCgGACUgccugGGGCUCUGc -5' |
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28564 | 5' | -62.1 | NC_005946.1 | + | 47410 | 0.71 | 0.289279 |
Target: 5'- uGCgCCGGCCacucuggGGCGGgcuugaACCCCGGGAUGg -3' miRNA: 3'- -UGgGGUCGGa------CUGCC------UGGGGCUCUGC- -5' |
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28564 | 5' | -62.1 | NC_005946.1 | + | 13744 | 0.71 | 0.289279 |
Target: 5'- cGCCUCGGCCUGGgaccaGGACgCCgGGGACc -3' miRNA: 3'- -UGGGGUCGGACUg----CCUG-GGgCUCUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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