Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28564 | 5' | -62.1 | NC_005946.1 | + | 100886 | 0.72 | 0.270008 |
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Target: 5'- --gCCGGUCUGACGGcuGCCaUCGAGACGg -3' miRNA: 3'- uggGGUCGGACUGCC--UGG-GGCUCUGC- -5' |
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| 28564 | 5' | -62.1 | NC_005946.1 | + | 59667 | 0.72 | 0.263819 |
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Target: 5'- aGCCCUcuauCUUGACGGGCCUgGGGACGu -3' miRNA: 3'- -UGGGGuc--GGACUGCCUGGGgCUCUGC- -5' |
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| 28564 | 5' | -62.1 | NC_005946.1 | + | 64137 | 0.73 | 0.213203 |
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Target: 5'- gACCCgAGCUUGggcACGGACCCCGA-ACa -3' miRNA: 3'- -UGGGgUCGGAC---UGCCUGGGGCUcUGc -5' |
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| 28564 | 5' | -62.1 | NC_005946.1 | + | 67604 | 0.74 | 0.179369 |
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Target: 5'- uCCCCAGCCUGAUccacccgcaagugGGAagguCCCUGAGGCu -3' miRNA: 3'- uGGGGUCGGACUG-------------CCU----GGGGCUCUGc -5' |
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| 28564 | 5' | -62.1 | NC_005946.1 | + | 20718 | 0.75 | 0.175437 |
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Target: 5'- gUCaCCAGCCUGGCcGGCCUCGAGGCc -3' miRNA: 3'- uGG-GGUCGGACUGcCUGGGGCUCUGc -5' |
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| 28564 | 5' | -62.1 | NC_005946.1 | + | 35134 | 0.77 | 0.111512 |
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Target: 5'- cACUCaAGCCUGACGGACCUCaGGACGu -3' miRNA: 3'- -UGGGgUCGGACUGCCUGGGGcUCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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