Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28564 | 5' | -62.1 | NC_005946.1 | + | 13744 | 0.71 | 0.289279 |
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Target: 5'- cGCCUCGGCCUGGgaccaGGACgCCgGGGACc -3' miRNA: 3'- -UGGGGUCGGACUg----CCUG-GGgCUCUGc -5' |
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| 28564 | 5' | -62.1 | NC_005946.1 | + | 94806 | 0.7 | 0.361219 |
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Target: 5'- aACCCCAGgCUGAauauaCCCGAGACu -3' miRNA: 3'- -UGGGGUCgGACUgccugGGGCUCUGc -5' |
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| 28564 | 5' | -62.1 | NC_005946.1 | + | 26766 | 0.69 | 0.385098 |
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Target: 5'- uACCCCAGCUaGGgGGAgucucgacguCCCCGaAGGCa -3' miRNA: 3'- -UGGGGUCGGaCUgCCU----------GGGGC-UCUGc -5' |
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| 28564 | 5' | -62.1 | NC_005946.1 | + | 23316 | 0.68 | 0.418518 |
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Target: 5'- cGCCCCGGCCUuACGGuCCUCaGGAa- -3' miRNA: 3'- -UGGGGUCGGAcUGCCuGGGGcUCUgc -5' |
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| 28564 | 5' | -62.1 | NC_005946.1 | + | 12603 | 0.68 | 0.433246 |
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Target: 5'- uGCCCCAGgagguggaggagcuCCUGACGGugUCUcccgugGAGACc -3' miRNA: 3'- -UGGGGUC--------------GGACUGCCugGGG------CUCUGc -5' |
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| 28564 | 5' | -62.1 | NC_005946.1 | + | 36682 | 0.66 | 0.577192 |
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Target: 5'- aGCCUCucuCCUGACGGGCCUgGgcucGGACu -3' miRNA: 3'- -UGGGGuc-GGACUGCCUGGGgC----UCUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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