Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28564 | 5' | -62.1 | NC_005946.1 | + | 42360 | 0.66 | 0.537962 |
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Target: 5'- gACCCUuugGGCCgu-CGGGCCCCGcGuCGu -3' miRNA: 3'- -UGGGG---UCGGacuGCCUGGGGCuCuGC- -5' |
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| 28564 | 5' | -62.1 | NC_005946.1 | + | 9073 | 0.66 | 0.547693 |
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Target: 5'- -aCCUGGCCUGG-GGACCCugCGGGAgGu -3' miRNA: 3'- ugGGGUCGGACUgCCUGGG--GCUCUgC- -5' |
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| 28564 | 5' | -62.1 | NC_005946.1 | + | 27437 | 0.66 | 0.547693 |
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Target: 5'- cGCCCuCGGCCgaGAUGaGGCuCCUGAGGuCGg -3' miRNA: 3'- -UGGG-GUCGGa-CUGC-CUG-GGGCUCU-GC- -5' |
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| 28564 | 5' | -62.1 | NC_005946.1 | + | 72200 | 0.66 | 0.577192 |
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Target: 5'- uACCCCcaaaagAGCCUGGUGGACUCUagguccguaaaGAGGCu -3' miRNA: 3'- -UGGGG------UCGGACUGCCUGGGG-----------CUCUGc -5' |
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| 28564 | 5' | -62.1 | NC_005946.1 | + | 49439 | 0.66 | 0.577192 |
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Target: 5'- aACCCCuccagGGCCcuggagggGACGGGguCCUCGuAGACGg -3' miRNA: 3'- -UGGGG-----UCGGa-------CUGCCU--GGGGC-UCUGC- -5' |
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| 28564 | 5' | -62.1 | NC_005946.1 | + | 36682 | 0.66 | 0.577192 |
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Target: 5'- aGCCUCucuCCUGACGGGCCUgGgcucGGACu -3' miRNA: 3'- -UGGGGuc-GGACUGCCUGGGgC----UCUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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