Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28567 | 3' | -53 | NC_005946.1 | + | 94724 | 0.72 | 0.663851 |
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Target: 5'- gCAcCGUCcucccagAGGGuUCCAcGGGGGCCCUCa -3' miRNA: 3'- -GUuGCAG-------UUUC-AGGUuCCUCCGGGAG- -5' |
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| 28567 | 3' | -53 | NC_005946.1 | + | 26309 | 0.73 | 0.621861 |
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Target: 5'- -cACGUCAAAGUCCAGGGugaguGGagUCUCu -3' miRNA: 3'- guUGCAGUUUCAGGUUCCu----CCg-GGAG- -5' |
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| 28567 | 3' | -53 | NC_005946.1 | + | 20927 | 0.74 | 0.547144 |
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Target: 5'- aCGACGugcUCGGAGgcaaggCCcAGGAGGCCCUg -3' miRNA: 3'- -GUUGC---AGUUUCa-----GGuUCCUCCGGGAg -5' |
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| 28567 | 3' | -53 | NC_005946.1 | + | 24358 | 0.74 | 0.547144 |
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Target: 5'- aCAGCcuGUgGAAG-CCGggcuGGGAGGCCCUCu -3' miRNA: 3'- -GUUG--CAgUUUCaGGU----UCCUCCGGGAG- -5' |
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| 28567 | 3' | -53 | NC_005946.1 | + | 57877 | 0.74 | 0.536667 |
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Target: 5'- gAGCGcccaCAAGGUCaaCGGGGAGGCCUUCu -3' miRNA: 3'- gUUGCa---GUUUCAG--GUUCCUCCGGGAG- -5' |
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| 28567 | 3' | -53 | NC_005946.1 | + | 27840 | 0.79 | 0.30916 |
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Target: 5'- -----aCAAAGUCCAcGGGGGCCCUCc -3' miRNA: 3'- guugcaGUUUCAGGUuCCUCCGGGAG- -5' |
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| 28567 | 3' | -53 | NC_005946.1 | + | 47739 | 0.79 | 0.30916 |
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Target: 5'- aGGCGUCAGGGUCacucaCGAGGAGGUCCg- -3' miRNA: 3'- gUUGCAGUUUCAG-----GUUCCUCCGGGag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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