Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28569 | 3' | -40.4 | NC_005946.1 | + | 33686 | 0.68 | 1 |
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Target: 5'- ---uGCACGCAAAGAUGU----AGACu -3' miRNA: 3'- aaagUGUGUGUUUCUAUAaaugUCUG- -5' |
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| 28569 | 3' | -40.4 | NC_005946.1 | + | 15440 | 0.68 | 1 |
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Target: 5'- -aUCgGCAUaagagugaggACGAGGAcGUUUGCAGACa -3' miRNA: 3'- aaAG-UGUG----------UGUUUCUaUAAAUGUCUG- -5' |
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| 28569 | 3' | -40.4 | NC_005946.1 | + | 94320 | 0.66 | 1 |
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Target: 5'- -aUCAgGCugAGGGAgggcaggUACAGGCc -3' miRNA: 3'- aaAGUgUGugUUUCUauaa---AUGUCUG- -5' |
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| 28569 | 3' | -40.4 | NC_005946.1 | + | 7507 | 0.66 | 1 |
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Target: 5'- aUUUUuCGCAguAGGAcuccUGUUUGCGGGCa -3' miRNA: 3'- -AAAGuGUGUguUUCU----AUAAAUGUCUG- -5' |
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| 28569 | 3' | -40.4 | NC_005946.1 | + | 59413 | 0.69 | 0.999999 |
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Target: 5'- -aUgGCGCACGAacugaGGAUGUUUGCAaACu -3' miRNA: 3'- aaAgUGUGUGUU-----UCUAUAAAUGUcUG- -5' |
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| 28569 | 3' | -40.4 | NC_005946.1 | + | 63477 | 0.71 | 0.999951 |
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Target: 5'- --cCACguGCACAAAGAUGUc-ACAGGCc -3' miRNA: 3'- aaaGUG--UGUGUUUCUAUAaaUGUCUG- -5' |
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| 28569 | 3' | -40.4 | NC_005946.1 | + | 101369 | 0.72 | 0.999909 |
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Target: 5'- -aUUAgACACAAGGGUAUUgucaaccCAGACa -3' miRNA: 3'- aaAGUgUGUGUUUCUAUAAau-----GUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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