Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
2870 | 5' | -53.8 | NC_001493.1 | + | 48634 | 0.67 | 0.922609 |
Target: 5'- gGGAUaGCG-CGGCGAUcGUGgGUGGCa -3' miRNA: 3'- -CCUGcUGCuGCCGCUAcUAC-UACCGa -5' |
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2870 | 5' | -53.8 | NC_001493.1 | + | 109081 | 0.67 | 0.938236 |
Target: 5'- cGACGGCugucGACGGCGGauucaucggguUGAUGGgaaagugucUGGCg -3' miRNA: 3'- cCUGCUG----CUGCCGCU-----------ACUACU---------ACCGa -5' |
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2870 | 5' | -53.8 | NC_001493.1 | + | 114581 | 0.67 | 0.938236 |
Target: 5'- cGGAUGGCGuGCGGaCGGaGGUGuGUGGUUu -3' miRNA: 3'- -CCUGCUGC-UGCC-GCUaCUAC-UACCGA- -5' |
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2870 | 5' | -53.8 | NC_001493.1 | + | 61872 | 0.66 | 0.963004 |
Target: 5'- gGGAaGGCGAUGGCGAgcccGGUcGGCa -3' miRNA: 3'- -CCUgCUGCUGCCGCUacuaCUA-CCGa -5' |
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2870 | 5' | -53.8 | NC_001493.1 | + | 124704 | 0.66 | 0.955684 |
Target: 5'- cGGgGAUGGCGGCGAggGggGA-GGCc -3' miRNA: 3'- cCUgCUGCUGCCGCUa-CuaCUaCCGa -5' |
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2870 | 5' | -53.8 | NC_001493.1 | + | 9149 | 0.66 | 0.955684 |
Target: 5'- cGGgGAUGGCGGCGAggGggGA-GGCc -3' miRNA: 3'- cCUgCUGCUGCCGCUa-CuaCUaCCGa -5' |
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2870 | 5' | -53.8 | NC_001493.1 | + | 55705 | 0.66 | 0.955684 |
Target: 5'- aGGGCGGaGAUGGCGAaacuacgGGUGAcgGGUUu -3' miRNA: 3'- -CCUGCUgCUGCCGCUa------CUACUa-CCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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