Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2872 | 3' | -55.7 | NC_001493.1 | + | 3766 | 0.68 | 0.813128 |
Target: 5'- gAGUGGGUGgagaaGGAGggggcgagGCGCGGCGgUUu -3' miRNA: 3'- gUCGCCCACa----CCUUa-------UGCGCUGCgAG- -5' |
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2872 | 3' | -55.7 | NC_001493.1 | + | 119320 | 0.68 | 0.813128 |
Target: 5'- gAGUGGGUGgagaaGGAGggggcgagGCGCGGCGgUUu -3' miRNA: 3'- gUCGCCCACa----CCUUa-------UGCGCUGCgAG- -5' |
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2872 | 3' | -55.7 | NC_001493.1 | + | 37207 | 0.67 | 0.830433 |
Target: 5'- -cGCGGGUGaaGAGUuuCGCGugGCa- -3' miRNA: 3'- guCGCCCACacCUUAu-GCGCugCGag -5' |
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2872 | 3' | -55.7 | NC_001493.1 | + | 83864 | 0.66 | 0.870332 |
Target: 5'- uCGGauaGGGgagGUGGccguGUucguccggcGCGCGGCGCUCc -3' miRNA: 3'- -GUCg--CCCa--CACCu---UA---------UGCGCUGCGAG- -5' |
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2872 | 3' | -55.7 | NC_001493.1 | + | 26622 | 0.66 | 0.904784 |
Target: 5'- -uGCGGGUGaGGAGggcccccaaGCGACugguGCUCg -3' miRNA: 3'- guCGCCCACaCCUUaug------CGCUG----CGAG- -5' |
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2872 | 3' | -55.7 | NC_001493.1 | + | 88331 | 0.7 | 0.67725 |
Target: 5'- -uGCGGGUGUGGA---UGUGG-GCUCa -3' miRNA: 3'- guCGCCCACACCUuauGCGCUgCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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