Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2872 | 3' | -55.7 | NC_001493.1 | + | 10106 | 0.67 | 0.830433 |
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Target: 5'- gCGGUGGuuuGUGaUGGu---CGCGGCGCUCc -3' miRNA: 3'- -GUCGCC---CAC-ACCuuauGCGCUGCGAG- -5' |
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| 2872 | 3' | -55.7 | NC_001493.1 | + | 110896 | 0.67 | 0.838812 |
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Target: 5'- aGGUGGGUGcucGAAUGCGUGcCGCa- -3' miRNA: 3'- gUCGCCCACac-CUUAUGCGCuGCGag -5' |
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| 2872 | 3' | -55.7 | NC_001493.1 | + | 69475 | 0.67 | 0.846998 |
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Target: 5'- aCAGCGGGUGcacuGUACG-GGCGCg- -3' miRNA: 3'- -GUCGCCCACaccuUAUGCgCUGCGag -5' |
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| 2872 | 3' | -55.7 | NC_001493.1 | + | 53403 | 0.66 | 0.868085 |
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Target: 5'- aAGCGGGUGaaaaucgaggcgGGAGUacggggguaucuuACGCGGuugUGCUCg -3' miRNA: 3'- gUCGCCCACa-----------CCUUA-------------UGCGCU---GCGAG- -5' |
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| 2872 | 3' | -55.7 | NC_001493.1 | + | 83864 | 0.66 | 0.870332 |
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Target: 5'- uCGGauaGGGgagGUGGccguGUucguccggcGCGCGGCGCUCc -3' miRNA: 3'- -GUCg--CCCa--CACCu---UA---------UGCGCUGCGAG- -5' |
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| 2872 | 3' | -55.7 | NC_001493.1 | + | 26622 | 0.66 | 0.904784 |
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Target: 5'- -uGCGGGUGaGGAGggcccccaaGCGACugguGCUCg -3' miRNA: 3'- guCGCCCACaCCUUaug------CGCUG----CGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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