Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2872 | 3' | -55.7 | NC_001493.1 | + | 133579 | 0.71 | 0.625574 |
Target: 5'- gAGCGGGccacgGUGGAcauuUGCGCGACcaucCUCg -3' miRNA: 3'- gUCGCCCa----CACCUu---AUGCGCUGc---GAG- -5' |
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2872 | 3' | -55.7 | NC_001493.1 | + | 18025 | 0.71 | 0.625574 |
Target: 5'- gAGCGGGccacgGUGGAcauuUGCGCGACcaucCUCg -3' miRNA: 3'- gUCGCCCa----CACCUu---AUGCGCUGc---GAG- -5' |
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2872 | 3' | -55.7 | NC_001493.1 | + | 65707 | 0.72 | 0.569924 |
Target: 5'- gGGCGGGUGUGGccagaagaaGC-ACGCUCg -3' miRNA: 3'- gUCGCCCACACCuuaug----CGcUGCGAG- -5' |
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2872 | 3' | -55.7 | NC_001493.1 | + | 39668 | 0.75 | 0.393336 |
Target: 5'- -cGCGGGUcugGUGGGAUccgGCGUGACGCg- -3' miRNA: 3'- guCGCCCA---CACCUUA---UGCGCUGCGag -5' |
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2872 | 3' | -55.7 | NC_001493.1 | + | 4444 | 1.09 | 0.002728 |
Target: 5'- aCAGCGGGUGUGGAAUACGCGACGCUCc -3' miRNA: 3'- -GUCGCCCACACCUUAUGCGCUGCGAG- -5' |
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2872 | 3' | -55.7 | NC_001493.1 | + | 119998 | 1.09 | 0.002728 |
Target: 5'- aCAGCGGGUGUGGAAUACGCGACGCUCc -3' miRNA: 3'- -GUCGCCCACACCUUAUGCGCUGCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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