Results 41 - 43 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28724 | 3' | -53.6 | NC_006146.1 | + | 114382 | 0.74 | 0.63322 |
Target: 5'- uCAGCUGCU-GAUGGCcggCGACGACc -3' miRNA: 3'- uGUCGACGGuCUACCGua-GCUGUUGc -5' |
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28724 | 3' | -53.6 | NC_006146.1 | + | 27356 | 0.77 | 0.471432 |
Target: 5'- cGCGGCaagGCCGGggGGCGUCugGACAGCa -3' miRNA: 3'- -UGUCGa--CGGUCuaCCGUAG--CUGUUGc -5' |
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28724 | 3' | -53.6 | NC_006146.1 | + | 170157 | 1.09 | 0.004611 |
Target: 5'- gACAGCUGCCAGAUGGCAUCGACAACGa -3' miRNA: 3'- -UGUCGACGGUCUACCGUAGCUGUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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