Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28725 | 5' | -60.5 | NC_006146.1 | + | 109479 | 0.66 | 0.720349 |
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Target: 5'- aGCCCGGCcGUG-CCCGCguUGGc-- -3' miRNA: 3'- gUGGGCCGaCACgGGGCGguACUaga -5' |
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| 28725 | 5' | -60.5 | NC_006146.1 | + | 112339 | 0.67 | 0.685341 |
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Target: 5'- gAUCUGGCUGUGCCagggcuccauguaggCCGCCAcgcUGAc-- -3' miRNA: 3'- gUGGGCCGACACGG---------------GGCGGU---ACUaga -5' |
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| 28725 | 5' | -60.5 | NC_006146.1 | + | 113866 | 0.68 | 0.582451 |
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Target: 5'- aACCCuggcGGCUucGCCCCGgCCGUGAUUc -3' miRNA: 3'- gUGGG----CCGAcaCGGGGC-GGUACUAGa -5' |
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| 28725 | 5' | -60.5 | NC_006146.1 | + | 114529 | 0.74 | 0.287996 |
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Target: 5'- uUACCCGGCggcgGUGCUCCGCCAc----- -3' miRNA: 3'- -GUGGGCCGa---CACGGGGCGGUacuaga -5' |
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| 28725 | 5' | -60.5 | NC_006146.1 | + | 127861 | 0.66 | 0.729927 |
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Target: 5'- gCAgCCGGC-GUGCCauaCCGCgCGUGAUa- -3' miRNA: 3'- -GUgGGCCGaCACGG---GGCG-GUACUAga -5' |
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| 28725 | 5' | -60.5 | NC_006146.1 | + | 128063 | 0.66 | 0.739425 |
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Target: 5'- gGCCUGGCg--GCCCUcgagGCCcUGAUCc -3' miRNA: 3'- gUGGGCCGacaCGGGG----CGGuACUAGa -5' |
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| 28725 | 5' | -60.5 | NC_006146.1 | + | 156775 | 0.72 | 0.398983 |
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Target: 5'- aCACCUGGCUGUagGCCgCCGCCAg----- -3' miRNA: 3'- -GUGGGCCGACA--CGG-GGCGGUacuaga -5' |
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| 28725 | 5' | -60.5 | NC_006146.1 | + | 165244 | 0.67 | 0.638882 |
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Target: 5'- gCACCCgcGGCUgGUGCucgucccccuguccCCCGCCAUGGc-- -3' miRNA: 3'- -GUGGG--CCGA-CACG--------------GGGCGGUACUaga -5' |
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| 28725 | 5' | -60.5 | NC_006146.1 | + | 169071 | 1.06 | 0.001858 |
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Target: 5'- gCACCCGGCUGUGCCCCGCCAUGAUCUc -3' miRNA: 3'- -GUGGGCCGACACGGGGCGGUACUAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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