Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28725 | 5' | -60.5 | NC_006146.1 | + | 43408 | 0.66 | 0.710701 |
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Target: 5'- -cCCCGGCUGcgacCCCCGCCGUc---- -3' miRNA: 3'- guGGGCCGACac--GGGGCGGUAcuaga -5' |
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| 28725 | 5' | -60.5 | NC_006146.1 | + | 33130 | 0.66 | 0.710701 |
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Target: 5'- uCGCCgGGCcGcUGCCCCGCuCcgGGUg- -3' miRNA: 3'- -GUGGgCCGaC-ACGGGGCG-GuaCUAga -5' |
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| 28725 | 5' | -60.5 | NC_006146.1 | + | 109479 | 0.66 | 0.720349 |
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Target: 5'- aGCCCGGCcGUG-CCCGCguUGGc-- -3' miRNA: 3'- gUGGGCCGaCACgGGGCGguACUaga -5' |
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| 28725 | 5' | -60.5 | NC_006146.1 | + | 88529 | 0.66 | 0.729927 |
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Target: 5'- aACCCGGgUGUGgagCCCGCgAUGG-CUg -3' miRNA: 3'- gUGGGCCgACACg--GGGCGgUACUaGA- -5' |
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| 28725 | 5' | -60.5 | NC_006146.1 | + | 127861 | 0.66 | 0.729927 |
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Target: 5'- gCAgCCGGC-GUGCCauaCCGCgCGUGAUa- -3' miRNA: 3'- -GUgGGCCGaCACGG---GGCG-GUACUAga -5' |
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| 28725 | 5' | -60.5 | NC_006146.1 | + | 128063 | 0.66 | 0.739425 |
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Target: 5'- gGCCUGGCg--GCCCUcgagGCCcUGAUCc -3' miRNA: 3'- gUGGGCCGacaCGGGG----CGGuACUAGa -5' |
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| 28725 | 5' | -60.5 | NC_006146.1 | + | 53025 | 0.66 | 0.748835 |
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Target: 5'- uCACgCGGCUGgccgcgGCCgCCGCCGaGAg-- -3' miRNA: 3'- -GUGgGCCGACa-----CGG-GGCGGUaCUaga -5' |
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| 28725 | 5' | -60.5 | NC_006146.1 | + | 40956 | 0.66 | 0.748835 |
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Target: 5'- cCACCCcaGGUccGUGCCguagaCCGCCGUGGUg- -3' miRNA: 3'- -GUGGG--CCGa-CACGG-----GGCGGUACUAga -5' |
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| 28725 | 5' | -60.5 | NC_006146.1 | + | 4016 | 0.66 | 0.748835 |
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Target: 5'- aACgCGGCcuuUGCCCCGCuCGUGAa-- -3' miRNA: 3'- gUGgGCCGac-ACGGGGCG-GUACUaga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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