Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28725 | 5' | -60.5 | NC_006146.1 | + | 169071 | 1.06 | 0.001858 |
Target: 5'- gCACCCGGCUGUGCCCCGCCAUGAUCUc -3' miRNA: 3'- -GUGGGCCGACACGGGGCGGUACUAGA- -5' |
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28725 | 5' | -60.5 | NC_006146.1 | + | 93983 | 0.74 | 0.287996 |
Target: 5'- uCACCCGGCUGcaGCCCCGC---GGUCa -3' miRNA: 3'- -GUGGGCCGACa-CGGGGCGguaCUAGa -5' |
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28725 | 5' | -60.5 | NC_006146.1 | + | 114529 | 0.74 | 0.287996 |
Target: 5'- uUACCCGGCggcgGUGCUCCGCCAc----- -3' miRNA: 3'- -GUGGGCCGa---CACGGGGCGGUacuaga -5' |
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28725 | 5' | -60.5 | NC_006146.1 | + | 156775 | 0.72 | 0.398983 |
Target: 5'- aCACCUGGCUGUagGCCgCCGCCAg----- -3' miRNA: 3'- -GUGGGCCGACA--CGG-GGCGGUacuaga -5' |
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28725 | 5' | -60.5 | NC_006146.1 | + | 33288 | 0.7 | 0.477469 |
Target: 5'- gGCCCGGCUGggcaccgcUGCgCCGCCGcucGGUCc -3' miRNA: 3'- gUGGGCCGAC--------ACGgGGCGGUa--CUAGa -5' |
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28725 | 5' | -60.5 | NC_006146.1 | + | 33543 | 0.69 | 0.562847 |
Target: 5'- uGCCCGGCU-UGCCUgGCCcUGGUg- -3' miRNA: 3'- gUGGGCCGAcACGGGgCGGuACUAga -5' |
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28725 | 5' | -60.5 | NC_006146.1 | + | 113866 | 0.68 | 0.582451 |
Target: 5'- aACCCuggcGGCUucGCCCCGgCCGUGAUUc -3' miRNA: 3'- gUGGG----CCGAcaCGGGGC-GGUACUAGa -5' |
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28725 | 5' | -60.5 | NC_006146.1 | + | 33164 | 0.68 | 0.592306 |
Target: 5'- gGCCCGGCUGggcaccgccGCgCCGCCGcucGGUCc -3' miRNA: 3'- gUGGGCCGACa--------CGgGGCGGUa--CUAGa -5' |
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28725 | 5' | -60.5 | NC_006146.1 | + | 33689 | 0.68 | 0.599221 |
Target: 5'- cCACCCGGCuuccuccccggggUGUGCCCgGCCuacccccguUCUg -3' miRNA: 3'- -GUGGGCCG-------------ACACGGGgCGGuacu-----AGA- -5' |
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28725 | 5' | -60.5 | NC_006146.1 | + | 15433 | 0.68 | 0.602189 |
Target: 5'- gGCCCGGCUGcaGUCCUGCC-UGGc-- -3' miRNA: 3'- gUGGGCCGACa-CGGGGCGGuACUaga -5' |
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28725 | 5' | -60.5 | NC_006146.1 | + | 67801 | 0.68 | 0.62201 |
Target: 5'- gGCCaGGCUcUGCCCCGCCucgGAa-- -3' miRNA: 3'- gUGGgCCGAcACGGGGCGGua-CUaga -5' |
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28725 | 5' | -60.5 | NC_006146.1 | + | 165244 | 0.67 | 0.638882 |
Target: 5'- gCACCCgcGGCUgGUGCucgucccccuguccCCCGCCAUGGc-- -3' miRNA: 3'- -GUGGG--CCGA-CACG--------------GGGCGGUACUaga -5' |
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28725 | 5' | -60.5 | NC_006146.1 | + | 33876 | 0.67 | 0.640867 |
Target: 5'- cCACCCGGCcuccuccccggggUGUGCCCgGCCu------ -3' miRNA: 3'- -GUGGGCCG-------------ACACGGGgCGGuacuaga -5' |
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28725 | 5' | -60.5 | NC_006146.1 | + | 23735 | 0.67 | 0.651777 |
Target: 5'- gGCgCCGGuCUGcGCCgCCGCCG-GGUCa -3' miRNA: 3'- gUG-GGCC-GACaCGG-GGCGGUaCUAGa -5' |
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28725 | 5' | -60.5 | NC_006146.1 | + | 45912 | 0.67 | 0.66168 |
Target: 5'- gCGCCCGGCcgGUGaCCgUGCCGUagguggacggGGUCUc -3' miRNA: 3'- -GUGGGCCGa-CAC-GGgGCGGUA----------CUAGA- -5' |
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28725 | 5' | -60.5 | NC_006146.1 | + | 32937 | 0.67 | 0.66168 |
Target: 5'- -cCCCGGgaG-GCCCgGCCGcGAUCc -3' miRNA: 3'- guGGGCCgaCaCGGGgCGGUaCUAGa -5' |
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28725 | 5' | -60.5 | NC_006146.1 | + | 85489 | 0.67 | 0.66168 |
Target: 5'- gGCCUGGCUGgucuCCUCGUCggGAUCc -3' miRNA: 3'- gUGGGCCGACac--GGGGCGGuaCUAGa -5' |
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28725 | 5' | -60.5 | NC_006146.1 | + | 112339 | 0.67 | 0.685341 |
Target: 5'- gAUCUGGCUGUGCCagggcuccauguaggCCGCCAcgcUGAc-- -3' miRNA: 3'- gUGGGCCGACACGG---------------GGCGGU---ACUaga -5' |
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28725 | 5' | -60.5 | NC_006146.1 | + | 33376 | 0.67 | 0.691223 |
Target: 5'- cCGCCgGGCcGcUGCCCCGCuCcgGGUg- -3' miRNA: 3'- -GUGGgCCGaC-ACGGGGCG-GuaCUAga -5' |
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28725 | 5' | -60.5 | NC_006146.1 | + | 33254 | 0.67 | 0.691223 |
Target: 5'- cCGCCgGGCcGcUGCCCCGCuCcgGGUg- -3' miRNA: 3'- -GUGGgCCGaC-ACGGGGCG-GuaCUAga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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