Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28727 | 3' | -53.1 | NC_006146.1 | + | 152896 | 0.7 | 0.855578 |
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Target: 5'- -gGGUGGCGAUGCcGUC--CAGCUCGa -3' miRNA: 3'- cgUCGCCGCUGUGaUAGuaGUCGAGC- -5' |
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| 28727 | 3' | -53.1 | NC_006146.1 | + | 156928 | 0.67 | 0.963296 |
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Target: 5'- -gGGUGGCGGCGCUGaCG--GGCUCc -3' miRNA: 3'- cgUCGCCGCUGUGAUaGUagUCGAGc -5' |
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| 28727 | 3' | -53.1 | NC_006146.1 | + | 162431 | 0.66 | 0.982142 |
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Target: 5'- gGUAGCGGgaGAgaaaGCUAUCcaucuguuuUCAGCUCGc -3' miRNA: 3'- -CGUCGCCg-CUg---UGAUAGu--------AGUCGAGC- -5' |
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| 28727 | 3' | -53.1 | NC_006146.1 | + | 167316 | 0.69 | 0.898218 |
|
Target: 5'- uGCAGggggggcCGGCGGgGCgucccGUCGUCAcGCUCGg -3' miRNA: 3'- -CGUC-------GCCGCUgUGa----UAGUAGU-CGAGC- -5' |
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| 28727 | 3' | -53.1 | NC_006146.1 | + | 168248 | 0.69 | 0.898218 |
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Target: 5'- uGCAGggggggcCGGCGGgGCgucccGUCGUCAcGCUCGg -3' miRNA: 3'- -CGUC-------GCCGCUgUGa----UAGUAGU-CGAGC- -5' |
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| 28727 | 3' | -53.1 | NC_006146.1 | + | 168390 | 1.11 | 0.004395 |
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Target: 5'- cGCAGCGGCGACACUAUCAUCAGCUCGu -3' miRNA: 3'- -CGUCGCCGCUGUGAUAGUAGUCGAGC- -5' |
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| 28727 | 3' | -53.1 | NC_006146.1 | + | 169180 | 0.69 | 0.898218 |
|
Target: 5'- uGCAGggggggcCGGCGGgGCgucccGUCGUCAcGCUCGg -3' miRNA: 3'- -CGUC-------GCCGCUgUGa----UAGUAGU-CGAGC- -5' |
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| 28727 | 3' | -53.1 | NC_006146.1 | + | 170111 | 0.7 | 0.877512 |
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Target: 5'- cGCAGggggggcCGGCGGgGCgucccGUCGUCAcGCUCGg -3' miRNA: 3'- -CGUC-------GCCGCUgUGa----UAGUAGU-CGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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