Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28727 | 3' | -53.1 | NC_006146.1 | + | 77431 | 0.67 | 0.952023 |
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Target: 5'- uGCGGCaucCGAgACUAUggCAUCAGCUCc -3' miRNA: 3'- -CGUCGcc-GCUgUGAUA--GUAGUCGAGc -5' |
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| 28727 | 3' | -53.1 | NC_006146.1 | + | 75968 | 0.67 | 0.966603 |
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Target: 5'- cGCAGCGGCcACGucccCUgcAUCGUCugcgugGGCUCGc -3' miRNA: 3'- -CGUCGCCGcUGU----GA--UAGUAG------UCGAGC- -5' |
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| 28727 | 3' | -53.1 | NC_006146.1 | + | 57373 | 0.66 | 0.977736 |
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Target: 5'- uGCGGCGGUGGCGCcg--GUgGGCUgGc -3' miRNA: 3'- -CGUCGCCGCUGUGauagUAgUCGAgC- -5' |
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| 28727 | 3' | -53.1 | NC_006146.1 | + | 53859 | 0.69 | 0.917376 |
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Target: 5'- gGCGGUaGGCGACGCUGgcccUCAGCg-- -3' miRNA: 3'- -CGUCG-CCGCUGUGAUagu-AGUCGagc -5' |
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| 28727 | 3' | -53.1 | NC_006146.1 | + | 53166 | 0.66 | 0.982142 |
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Target: 5'- uGCGGCGGUGGC-Cggaa---GGCUCGg -3' miRNA: 3'- -CGUCGCCGCUGuGauaguagUCGAGC- -5' |
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| 28727 | 3' | -53.1 | NC_006146.1 | + | 49499 | 0.66 | 0.982142 |
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Target: 5'- aGgGGCGGCGugGC-AUCuggAUCGGCg-- -3' miRNA: 3'- -CgUCGCCGCugUGaUAG---UAGUCGagc -5' |
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| 28727 | 3' | -53.1 | NC_006146.1 | + | 46292 | 0.67 | 0.966603 |
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Target: 5'- cCGGUGGCGGCG--GUCG--AGCUCGa -3' miRNA: 3'- cGUCGCCGCUGUgaUAGUagUCGAGC- -5' |
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| 28727 | 3' | -53.1 | NC_006146.1 | + | 45180 | 0.67 | 0.969694 |
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Target: 5'- uCGGCGGCGGCGCUccccuccUCAUCuucCUCc -3' miRNA: 3'- cGUCGCCGCUGUGAu------AGUAGuc-GAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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