Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28735 | 5' | -46.6 | NC_006146.1 | + | 162799 | 0.76 | 0.888479 |
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Target: 5'- aUCCagauAAGUUcGGGUAGCAUAUGCUa -3' miRNA: 3'- cAGGau--UUCAAcCCUAUCGUAUACGA- -5' |
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| 28735 | 5' | -46.6 | NC_006146.1 | + | 163359 | 0.77 | 0.857036 |
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Target: 5'- aUCCUGAuauGUUaGGGUAGUAUAUGCUa -3' miRNA: 3'- cAGGAUUu--CAAcCCUAUCGUAUACGA- -5' |
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| 28735 | 5' | -46.6 | NC_006146.1 | + | 162880 | 0.84 | 0.535348 |
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Target: 5'- aUCUUAAuaUUGGGGUAGCAUAUGCUa -3' miRNA: 3'- cAGGAUUucAACCCUAUCGUAUACGA- -5' |
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| 28735 | 5' | -46.6 | NC_006146.1 | + | 163278 | 0.87 | 0.40468 |
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Target: 5'- aUCCUAAuuuAGUUGGG-UAGCAUAUGCUa -3' miRNA: 3'- cAGGAUU---UCAACCCuAUCGUAUACGA- -5' |
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| 28735 | 5' | -46.6 | NC_006146.1 | + | 163250 | 0.87 | 0.40468 |
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Target: 5'- aUCCUAAuuuAGUUGGG-UAGCAUAUGCUa -3' miRNA: 3'- cAGGAUU---UCAACCCuAUCGUAUACGA- -5' |
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| 28735 | 5' | -46.6 | NC_006146.1 | + | 7608 | 0.88 | 0.35151 |
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Target: 5'- -aCCUAAA-UUGGGAUAGCAUAUGCUa -3' miRNA: 3'- caGGAUUUcAACCCUAUCGUAUACGA- -5' |
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| 28735 | 5' | -46.6 | NC_006146.1 | + | 162933 | 1.09 | 0.021243 |
|
Target: 5'- uGUCCUAAAGUUGGGAUAGCAUAUGCUa -3' miRNA: 3'- -CAGGAUUUCAACCCUAUCGUAUACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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