Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28735 | 5' | -46.6 | NC_006146.1 | + | 43943 | 0.68 | 0.999457 |
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Target: 5'- uUUgaAAAGgcgcGGGAUAGUGUAUGCUa -3' miRNA: 3'- cAGgaUUUCaa--CCCUAUCGUAUACGA- -5' |
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| 28735 | 5' | -46.6 | NC_006146.1 | + | 139100 | 0.69 | 0.998449 |
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Target: 5'- -gCCUGGAGUUGGGuu-GCGUGggGUg -3' miRNA: 3'- caGGAUUUCAACCCuauCGUAUa-CGa -5' |
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| 28735 | 5' | -46.6 | NC_006146.1 | + | 8082 | 0.69 | 0.99805 |
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Target: 5'- -aCCUAAcuauaucuGGAUAGCAUAUGCUa -3' miRNA: 3'- caGGAUUucaac---CCUAUCGUAUACGA- -5' |
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| 28735 | 5' | -46.6 | NC_006146.1 | + | 9723 | 0.72 | 0.976616 |
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Target: 5'- -gCCUu-GGUcaucuucUGGGGUAGUAUAUGCUa -3' miRNA: 3'- caGGAuuUCA-------ACCCUAUCGUAUACGA- -5' |
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| 28735 | 5' | -46.6 | NC_006146.1 | + | 8138 | 0.74 | 0.948296 |
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Target: 5'- aUCCUAAuuGUUGG--UAGCGUAUGCUa -3' miRNA: 3'- cAGGAUUu-CAACCcuAUCGUAUACGA- -5' |
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| 28735 | 5' | -46.6 | NC_006146.1 | + | 7608 | 0.88 | 0.35151 |
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Target: 5'- -aCCUAAA-UUGGGAUAGCAUAUGCUa -3' miRNA: 3'- caGGAUUUcAACCCUAUCGUAUACGA- -5' |
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| 28735 | 5' | -46.6 | NC_006146.1 | + | 7494 | 0.66 | 0.999947 |
|
Target: 5'- -cCCaaUAuAGUa-GGAUAGCAUAUGCUa -3' miRNA: 3'- caGG--AUuUCAacCCUAUCGUAUACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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