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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28736 | 5' | -44.2 | NC_006146.1 | + | 163236 | 0.92 | 0.302491 |
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Target: 5'- --cGGUAGCAUAUGCUAUCCUAAUu- -3' miRNA: 3'- aauCCAUCGUAUACGAUAGGAUUAua -5' |
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| 28736 | 5' | -44.2 | NC_006146.1 | + | 162918 | 0.92 | 0.287137 |
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Target: 5'- cUGGGUAGUAUAUGCUGUCCUAAa-- -3' miRNA: 3'- aAUCCAUCGUAUACGAUAGGAUUaua -5' |
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| 28736 | 5' | -44.2 | NC_006146.1 | + | 162812 | 0.92 | 0.279693 |
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Target: 5'- -cGGGUAGCAUAUGCUAUCCaGAUAUa -3' miRNA: 3'- aaUCCAUCGUAUACGAUAGGaUUAUA- -5' |
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| 28736 | 5' | -44.2 | NC_006146.1 | + | 162997 | 0.95 | 0.196491 |
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Target: 5'- cUGGGUAGCAUAUGCUAUCCUAGc-- -3' miRNA: 3'- aAUCCAUCGUAUACGAUAGGAUUaua -5' |
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| 28736 | 5' | -44.2 | NC_006146.1 | + | 163372 | 0.97 | 0.15674 |
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Target: 5'- -aGGGUAGUAUAUGCUAUCCUAGUGUa -3' miRNA: 3'- aaUCCAUCGUAUACGAUAGGAUUAUA- -5' |
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| 28736 | 5' | -44.2 | NC_006146.1 | + | 163075 | 0.98 | 0.148006 |
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Target: 5'- gUGGGUAGCAUAUGCUAUCCUAAUu- -3' miRNA: 3'- aAUCCAUCGUAUACGAUAGGAUUAua -5' |
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| 28736 | 5' | -44.2 | NC_006146.1 | + | 163261 | 0.98 | 0.143808 |
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Target: 5'- gUUGGGUAGCAUAUGCUAUCCUAAUu- -3' miRNA: 3'- -AAUCCAUCGUAUACGAUAGGAUUAua -5' |
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| 28736 | 5' | -44.2 | NC_006146.1 | + | 163342 | 0.99 | 0.124406 |
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Target: 5'- uUUGGGUAGCAUAUGUUAUCCUGAUAUg -3' miRNA: 3'- -AAUCCAUCGUAUACGAUAGGAUUAUA- -5' |
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| 28736 | 5' | -44.2 | NC_006146.1 | + | 162891 | 1 | 0.113959 |
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Target: 5'- -gGGGUAGCAUAUGCUAUCCUAGUAc -3' miRNA: 3'- aaUCCAUCGUAUACGAUAGGAUUAUa -5' |
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| 28736 | 5' | -44.2 | NC_006146.1 | + | 162838 | 1.04 | 0.068744 |
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Target: 5'- gUUAGGUAGCAUAUGCUAUCCUAAUAUu -3' miRNA: 3'- -AAUCCAUCGUAUACGAUAGGAUUAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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