Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2874 | 5' | -56.9 | NC_001493.1 | + | 124798 | 0.66 | 0.864972 |
|
Target: 5'- -aGGAUGCGUCgUCGgGAcaggaccGggGAGGCGg -3' miRNA: 3'- gcUCUGCGCGG-AGUgCU-------CuuCUCCGC- -5' |
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| 2874 | 5' | -56.9 | NC_001493.1 | + | 125678 | 0.67 | 0.817484 |
|
Target: 5'- gCGAuuCGCGaCCUCACGGGGccauacAGAGGg- -3' miRNA: 3'- -GCUcuGCGC-GGAGUGCUCU------UCUCCgc -5' |
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| 2874 | 5' | -56.9 | NC_001493.1 | + | 126202 | 0.66 | 0.842449 |
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Target: 5'- uCGGGACGCGCCcgaucgCGCGAGcuccccGGGUu -3' miRNA: 3'- -GCUCUGCGCGGa-----GUGCUCuuc---UCCGc -5' |
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| 2874 | 5' | -56.9 | NC_001493.1 | + | 126513 | 0.66 | 0.873067 |
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Target: 5'- aCGAGauGCGCGCCUUcuACGAGAcGuGcGCc -3' miRNA: 3'- -GCUC--UGCGCGGAG--UGCUCUuCuC-CGc -5' |
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| 2874 | 5' | -56.9 | NC_001493.1 | + | 129031 | 0.68 | 0.791025 |
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Target: 5'- gGAGGCGUgcgccgucgaucGCCUCugcaucggggGgGGGAGGAGGCu -3' miRNA: 3'- gCUCUGCG------------CGGAG----------UgCUCUUCUCCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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