Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28740 | 3' | -54.8 | NC_006146.1 | + | 132782 | 0.7 | 0.761414 |
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Target: 5'- cGGUCGCUgCAGGCUCgcaGCUgcucagcagcugcaGGCGGGGGa -3' miRNA: 3'- -CUAGCGA-GUCUGAGa--CGG--------------UCGUCUCU- -5' |
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| 28740 | 3' | -54.8 | NC_006146.1 | + | 28402 | 0.79 | 0.290212 |
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Target: 5'- gGAUCGCcCGGGuCUCUGCUGGCAGAGu -3' miRNA: 3'- -CUAGCGaGUCU-GAGACGGUCGUCUCu -5' |
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| 28740 | 3' | -54.8 | NC_006146.1 | + | 25324 | 0.79 | 0.290212 |
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Target: 5'- gGAUCGCcCGGGuCUCUGCUGGCAGAGu -3' miRNA: 3'- -CUAGCGaGUCU-GAGACGGUCGUCUCu -5' |
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| 28740 | 3' | -54.8 | NC_006146.1 | + | 22246 | 0.79 | 0.290212 |
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Target: 5'- gGAUCGCcCGGGuCUCUGCUGGCAGAGu -3' miRNA: 3'- -CUAGCGaGUCU-GAGACGGUCGUCUCu -5' |
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| 28740 | 3' | -54.8 | NC_006146.1 | + | 19168 | 0.79 | 0.290212 |
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Target: 5'- gGAUCGCcCGGGuCUCUGCUGGCAGAGu -3' miRNA: 3'- -CUAGCGaGUCU-GAGACGGUCGUCUCu -5' |
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| 28740 | 3' | -54.8 | NC_006146.1 | + | 16090 | 0.79 | 0.290212 |
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Target: 5'- gGAUCGCcCGGGuCUCUGCUGGCAGAGu -3' miRNA: 3'- -CUAGCGaGUCU-GAGACGGUCGUCUCu -5' |
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| 28740 | 3' | -54.8 | NC_006146.1 | + | 13012 | 0.79 | 0.290212 |
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Target: 5'- gGAUCGCcCGGGuCUCUGCUGGCAGAGu -3' miRNA: 3'- -CUAGCGaGUCU-GAGACGGUCGUCUCu -5' |
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| 28740 | 3' | -54.8 | NC_006146.1 | + | 123596 | 0.66 | 0.937045 |
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Target: 5'- ---aGCUCAaagaaGCUCUuggccaGCCAGCGGAGGu -3' miRNA: 3'- cuagCGAGUc----UGAGA------CGGUCGUCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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