Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28759 | 3' | -44.8 | NC_006146.1 | + | 155787 | 0.66 | 0.999998 |
Target: 5'- -aUGGGuGGCUGUguguaGUGUgUGCCUGg -3' miRNA: 3'- gaAUUUuCCGACAac---CAUAgAUGGAC- -5' |
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28759 | 3' | -44.8 | NC_006146.1 | + | 152709 | 0.66 | 0.999998 |
Target: 5'- -aUGGGuGGCUGUguguaGUGUgUGCCUGg -3' miRNA: 3'- gaAUUUuCCGACAac---CAUAgAUGGAC- -5' |
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28759 | 3' | -44.8 | NC_006146.1 | + | 149632 | 0.66 | 0.999998 |
Target: 5'- -aUGGGuGGCUGUguguaGUGUgUGCCUGg -3' miRNA: 3'- gaAUUUuCCGACAac---CAUAgAUGGAC- -5' |
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28759 | 3' | -44.8 | NC_006146.1 | + | 146554 | 0.66 | 0.999998 |
Target: 5'- -aUGGGuGGCUGUguguaGUGUgUGCCUGg -3' miRNA: 3'- gaAUUUuCCGACAac---CAUAgAUGGAC- -5' |
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28759 | 3' | -44.8 | NC_006146.1 | + | 143476 | 0.66 | 0.999998 |
Target: 5'- -aUGGGuGGCUGUguguaGUGUgUGCCUGg -3' miRNA: 3'- gaAUUUuCCGACAac---CAUAgAUGGAC- -5' |
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28759 | 3' | -44.8 | NC_006146.1 | + | 97567 | 0.66 | 0.999998 |
Target: 5'- --gAAGGGGCUGUUuGcGaAUCUGCaCUGa -3' miRNA: 3'- gaaUUUUCCGACAA-C-CaUAGAUG-GAC- -5' |
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28759 | 3' | -44.8 | NC_006146.1 | + | 87407 | 0.66 | 0.999998 |
Target: 5'- --gGAGAGGCaccacgcuucUGUgaaugUGGU-UCUGCCUGa -3' miRNA: 3'- gaaUUUUCCG----------ACA-----ACCAuAGAUGGAC- -5' |
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28759 | 3' | -44.8 | NC_006146.1 | + | 35376 | 0.66 | 0.999998 |
Target: 5'- uUUGGAGGGCUGUUuGGUGcaacugaagCUGCUc- -3' miRNA: 3'- gAAUUUUCCGACAA-CCAUa--------GAUGGac -5' |
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28759 | 3' | -44.8 | NC_006146.1 | + | 134938 | 0.66 | 0.999995 |
Target: 5'- --aGAGAGGCUGgagGGUGauUCU-CUUGa -3' miRNA: 3'- gaaUUUUCCGACaa-CCAU--AGAuGGAC- -5' |
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28759 | 3' | -44.8 | NC_006146.1 | + | 90113 | 0.67 | 0.999988 |
Target: 5'- -aUGAGGGGCcuccucuggGGUAUCUccauGCCUGg -3' miRNA: 3'- gaAUUUUCCGacaa-----CCAUAGA----UGGAC- -5' |
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28759 | 3' | -44.8 | NC_006146.1 | + | 154117 | 0.68 | 0.999971 |
Target: 5'- --gAAGGGGCUGUUGGacuuggccaucaUGUCggugACCa- -3' miRNA: 3'- gaaUUUUCCGACAACC------------AUAGa---UGGac -5' |
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28759 | 3' | -44.8 | NC_006146.1 | + | 102392 | 0.69 | 0.999875 |
Target: 5'- ---cGGGGaGCUGgUGGUAUCUGucCCUGg -3' miRNA: 3'- gaauUUUC-CGACaACCAUAGAU--GGAC- -5' |
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28759 | 3' | -44.8 | NC_006146.1 | + | 53445 | 0.75 | 0.973435 |
Target: 5'- --gAAAGGGCgg-UGGUGUCUGCCc- -3' miRNA: 3'- gaaUUUUCCGacaACCAUAGAUGGac -5' |
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28759 | 3' | -44.8 | NC_006146.1 | + | 140381 | 1.09 | 0.035996 |
Target: 5'- gCUUAAAAGGCUGUUGGUAUCUACCUGc -3' miRNA: 3'- -GAAUUUUCCGACAACCAUAGAUGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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