Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28772 | 3' | -58.1 | NC_006146.1 | + | 100238 | 0.66 | 0.853724 |
Target: 5'- uCCgugGCaGUGGGGgcgUUCACGcacGGGGGCUc -3' miRNA: 3'- -GGa--CG-CACCCUa--AGGUGC---CCUCCGAu -5' |
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28772 | 3' | -58.1 | NC_006146.1 | + | 33237 | 0.66 | 0.845957 |
Target: 5'- --gGC-UGGGGUUCCGgGGGuggGGGCUc -3' miRNA: 3'- ggaCGcACCCUAAGGUgCCC---UCCGAu -5' |
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28772 | 3' | -58.1 | NC_006146.1 | + | 157597 | 0.66 | 0.838003 |
Target: 5'- cCCUGgugcccagcaCGUGGGGguagUCGCGGGcGGGCa- -3' miRNA: 3'- -GGAC----------GCACCCUaa--GGUGCCC-UCCGau -5' |
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28772 | 3' | -58.1 | NC_006146.1 | + | 39349 | 0.66 | 0.833144 |
Target: 5'- uCCUGCGauucuuuggauguucUGGG--UCC-CGGGAGGgUGa -3' miRNA: 3'- -GGACGC---------------ACCCuaAGGuGCCCUCCgAU- -5' |
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28772 | 3' | -58.1 | NC_006146.1 | + | 52912 | 0.67 | 0.81309 |
Target: 5'- gUCUGCGUGGccc-CC-CGGGGGcGCUGg -3' miRNA: 3'- -GGACGCACCcuaaGGuGCCCUC-CGAU- -5' |
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28772 | 3' | -58.1 | NC_006146.1 | + | 103376 | 0.67 | 0.810517 |
Target: 5'- aCgaGCGUGGGGgagauagggcgccugCCAgUGGGGGGCg- -3' miRNA: 3'- -GgaCGCACCCUaa-------------GGU-GCCCUCCGau -5' |
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28772 | 3' | -58.1 | NC_006146.1 | + | 53801 | 0.67 | 0.795678 |
Target: 5'- gCCcGCGc-GGcgUCCauaGCGGGGGGCUGg -3' miRNA: 3'- -GGaCGCacCCuaAGG---UGCCCUCCGAU- -5' |
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28772 | 3' | -58.1 | NC_006146.1 | + | 130491 | 0.67 | 0.790341 |
Target: 5'- cCCcGgGUGGcuacuuugcccCCGCGGGAGGCUAc -3' miRNA: 3'- -GGaCgCACCcuaa-------GGUGCCCUCCGAU- -5' |
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28772 | 3' | -58.1 | NC_006146.1 | + | 70698 | 0.67 | 0.786756 |
Target: 5'- uUCUGCGcUGGGAacgggagCUcaggaggaGCGGGGGGCUu -3' miRNA: 3'- -GGACGC-ACCCUaa-----GG--------UGCCCUCCGAu -5' |
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28772 | 3' | -58.1 | NC_006146.1 | + | 94690 | 0.67 | 0.786756 |
Target: 5'- aCCU-CG-GGGGUUCUugGGGuGGGCc- -3' miRNA: 3'- -GGAcGCaCCCUAAGGugCCC-UCCGau -5' |
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28772 | 3' | -58.1 | NC_006146.1 | + | 75249 | 0.68 | 0.759219 |
Target: 5'- aCCgaggcggGCGUGGGGUg-CACGGGGauGGCc- -3' miRNA: 3'- -GGa------CGCACCCUAagGUGCCCU--CCGau -5' |
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28772 | 3' | -58.1 | NC_006146.1 | + | 39512 | 0.68 | 0.755469 |
Target: 5'- gCCUGCuGUGGGuggcugaugggCUggGGGAGGCg- -3' miRNA: 3'- -GGACG-CACCCuaa--------GGugCCCUCCGau -5' |
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28772 | 3' | -58.1 | NC_006146.1 | + | 170078 | 0.68 | 0.749812 |
Target: 5'- --cGCGUGGGGauggCCgGCGGGAGGg-- -3' miRNA: 3'- ggaCGCACCCUaa--GG-UGCCCUCCgau -5' |
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28772 | 3' | -58.1 | NC_006146.1 | + | 169146 | 0.68 | 0.749812 |
Target: 5'- --cGCGUGGGGauggCCgGCGGGAGGg-- -3' miRNA: 3'- ggaCGCACCCUaa--GG-UGCCCUCCgau -5' |
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28772 | 3' | -58.1 | NC_006146.1 | + | 168214 | 0.68 | 0.749812 |
Target: 5'- --cGCGUGGGGauggCCgGCGGGAGGg-- -3' miRNA: 3'- ggaCGCACCCUaa--GG-UGCCCUCCgau -5' |
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28772 | 3' | -58.1 | NC_006146.1 | + | 167282 | 0.68 | 0.749812 |
Target: 5'- --cGCGUGGGGauggCCgGCGGGAGGg-- -3' miRNA: 3'- ggaCGCACCCUaa--GG-UGCCCUCCgau -5' |
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28772 | 3' | -58.1 | NC_006146.1 | + | 39745 | 0.69 | 0.70146 |
Target: 5'- gCUG-GUGGGuUUCgGCuGGAGGCUGg -3' miRNA: 3'- gGACgCACCCuAAGgUGcCCUCCGAU- -5' |
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28772 | 3' | -58.1 | NC_006146.1 | + | 58098 | 0.69 | 0.671706 |
Target: 5'- aUCUGUacGUGGGGcgCaACGGGAGGCa- -3' miRNA: 3'- -GGACG--CACCCUaaGgUGCCCUCCGau -5' |
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28772 | 3' | -58.1 | NC_006146.1 | + | 45335 | 0.69 | 0.671706 |
Target: 5'- gUUGCG-GGGAUUCgG-GGGAGGCc- -3' miRNA: 3'- gGACGCaCCCUAAGgUgCCCUCCGau -5' |
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28772 | 3' | -58.1 | NC_006146.1 | + | 159222 | 0.7 | 0.6517 |
Target: 5'- aUCUGCGU-GGAUaCCGUGGGGGGCc- -3' miRNA: 3'- -GGACGCAcCCUAaGGUGCCCUCCGau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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