Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28772 | 3' | -58.1 | NC_006146.1 | + | 103376 | 0.67 | 0.810517 |
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Target: 5'- aCgaGCGUGGGGgagauagggcgccugCCAgUGGGGGGCg- -3' miRNA: 3'- -GgaCGCACCCUaa-------------GGU-GCCCUCCGau -5' |
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| 28772 | 3' | -58.1 | NC_006146.1 | + | 52912 | 0.67 | 0.81309 |
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Target: 5'- gUCUGCGUGGccc-CC-CGGGGGcGCUGg -3' miRNA: 3'- -GGACGCACCcuaaGGuGCCCUC-CGAU- -5' |
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| 28772 | 3' | -58.1 | NC_006146.1 | + | 39349 | 0.66 | 0.833144 |
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Target: 5'- uCCUGCGauucuuuggauguucUGGG--UCC-CGGGAGGgUGa -3' miRNA: 3'- -GGACGC---------------ACCCuaAGGuGCCCUCCgAU- -5' |
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| 28772 | 3' | -58.1 | NC_006146.1 | + | 157597 | 0.66 | 0.838003 |
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Target: 5'- cCCUGgugcccagcaCGUGGGGguagUCGCGGGcGGGCa- -3' miRNA: 3'- -GGAC----------GCACCCUaa--GGUGCCC-UCCGau -5' |
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| 28772 | 3' | -58.1 | NC_006146.1 | + | 33237 | 0.66 | 0.845957 |
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Target: 5'- --gGC-UGGGGUUCCGgGGGuggGGGCUc -3' miRNA: 3'- ggaCGcACCCUAAGGUgCCC---UCCGAu -5' |
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| 28772 | 3' | -58.1 | NC_006146.1 | + | 100238 | 0.66 | 0.853724 |
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Target: 5'- uCCgugGCaGUGGGGgcgUUCACGcacGGGGGCUc -3' miRNA: 3'- -GGa--CG-CACCCUa--AGGUGC---CCUCCGAu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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