miRNA: 3'- -CUGCU-GACCUUgUGGGa--AGA-CCGGAG- -5'
miRNA: 3'- -CUGCUGa-----------CCUUGUGGGAa--GACCGGAG- -5'
miRNA: 3'- cUGCUGa------CCUUGUGGGaaGACCGGAG- -5'
miRNA: 3'- -CUGCUGACCUUGUGGGAAGACCGGAG- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28781 | 3' | -55.6 | NC_006146.1 | + | 57997 | 0.71 | 0.704288 |
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Target: 5'- gGACGAgCUGGAG-GCCCgggUCUcGGCCUUc -3' miRNA: 3'- -CUGCU-GACCUUgUGGGa--AGA-CCGGAG- -5' |
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| 28781 | 3' | -55.6 | NC_006146.1 | + | 103257 | 0.72 | 0.6104 |
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Target: 5'- gGACGAUagaugccuacagGGGGCGCCUUccaCUGGCCUCg -3' miRNA: 3'- -CUGCUGa-----------CCUUGUGGGAa--GACCGGAG- -5' |
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| 28781 | 3' | -55.6 | NC_006146.1 | + | 219 | 0.75 | 0.473493 |
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Target: 5'- cGCGACgguccccGGGGCGCCC--CUGGCCUCc -3' miRNA: 3'- cUGCUGa------CCUUGUGGGaaGACCGGAG- -5' |
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| 28781 | 3' | -55.6 | NC_006146.1 | + | 122301 | 1.09 | 0.003383 |
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Target: 5'- cGACGACUGGAACACCCUUCUGGCCUCc -3' miRNA: 3'- -CUGCUGACCUUGUGGGAAGACCGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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