miRNA: 3'- -CUGC---UGa-CCUUGUG-GGaa--GACCGGAG- -5'
miRNA: 3'- cugCUGACCUUgUGGGAaga-CCGGag -5'
miRNA: 3'- -CUGC--UGACCUUGUG-GGAAGa-CC----GGAG- -5'
miRNA: 3'- -CUGCUGA----------CCUUGUGGGaa-Ga--CCGGAg -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28781 | 3' | -55.6 | NC_006146.1 | + | 155577 | 0.67 | 0.887913 |
|
Target: 5'- gGGCGcccGCcaGGGGCACaCCggggCUGGCCUCc -3' miRNA: 3'- -CUGC---UGa-CCUUGUG-GGaa--GACCGGAG- -5' |
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| 28781 | 3' | -55.6 | NC_006146.1 | + | 157312 | 0.67 | 0.901137 |
|
Target: 5'- --gGACUGGAGgGCCCUgggaGGCCc- -3' miRNA: 3'- cugCUGACCUUgUGGGAaga-CCGGag -5' |
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| 28781 | 3' | -55.6 | NC_006146.1 | + | 159619 | 0.68 | 0.826369 |
|
Target: 5'- uGGCGcuGCUGGGGCACgCCUUCcgGGaacaCCUCc -3' miRNA: 3'- -CUGC--UGACCUUGUG-GGAAGa-CC----GGAG- -5' |
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| 28781 | 3' | -55.6 | NC_006146.1 | + | 161754 | 0.66 | 0.9074 |
|
Target: 5'- uGGCGGCUgagguccgagGGGGCGCCUgggCgggGGCCUg -3' miRNA: 3'- -CUGCUGA----------CCUUGUGGGaa-Ga--CCGGAg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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