miRNA: 3'- -CugCUGACcUUGugGGAagACCGGAG- -5'
miRNA: 3'- cugCUGA-CCu---UGUGGGAAGACCGGag -5'
miRNA: 3'- cUGC------UGACCUUGUG-GGAAGA----------CCGGag -5'
miRNA: 3'- cUGC-UGaCCUUgUGGGaaGACCGGAG- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28781 | 3' | -55.6 | NC_006146.1 | + | 39652 | 0.69 | 0.809068 |
|
Target: 5'- gGugGGCUGaGACugCCU--UGGCCUUg -3' miRNA: 3'- -CugCUGACcUUGugGGAagACCGGAG- -5' |
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| 28781 | 3' | -55.6 | NC_006146.1 | + | 24539 | 0.69 | 0.817803 |
|
Target: 5'- --aGGCUcGGuuucGCGCUCUUCUGGCCg- -3' miRNA: 3'- cugCUGA-CCu---UGUGGGAAGACCGGag -5' |
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| 28781 | 3' | -55.6 | NC_006146.1 | + | 52606 | 0.68 | 0.850972 |
|
Target: 5'- aGCGccauuuGCUGGAGCACuCCUUCUuccuggaccgGGCCg- -3' miRNA: 3'- cUGC------UGACCUUGUG-GGAAGA----------CCGGag -5' |
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| 28781 | 3' | -55.6 | NC_006146.1 | + | 15881 | 0.66 | 0.935141 |
|
Target: 5'- aGCGuGCcGGAGgGCUCg-CUGGCCUCg -3' miRNA: 3'- cUGC-UGaCCUUgUGGGaaGACCGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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