Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2882 | 5' | -56.2 | NC_001493.1 | + | 12970 | 0.66 | 0.902435 |
Target: 5'- gGGGC-CGgaCGGGUCCGgucacCACGUGGAg-- -3' miRNA: 3'- -CCUGcGC--GCCCAGGC-----GUGCAUCUaug -5' |
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2882 | 5' | -56.2 | NC_001493.1 | + | 47915 | 0.66 | 0.902435 |
Target: 5'- cGGACcCGCGGGUCUGgAuCGUcugGGGUuguACa -3' miRNA: 3'- -CCUGcGCGCCCAGGCgU-GCA---UCUA---UG- -5' |
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2882 | 5' | -56.2 | NC_001493.1 | + | 48063 | 0.66 | 0.902435 |
Target: 5'- aGACcCGCGGGUCCGacCGCG-AGcuccAUGCa -3' miRNA: 3'- cCUGcGCGCCCAGGC--GUGCaUC----UAUG- -5' |
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2882 | 5' | -56.2 | NC_001493.1 | + | 128524 | 0.66 | 0.902435 |
Target: 5'- gGGGC-CGgaCGGGUCCGgucacCACGUGGAg-- -3' miRNA: 3'- -CCUGcGC--GCCCAGGC-----GUGCAUCUaug -5' |
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2882 | 5' | -56.2 | NC_001493.1 | + | 40836 | 0.66 | 0.882522 |
Target: 5'- uGACGUGgaugaaaaacccCGGGUCCGagcCGUGGAcGCg -3' miRNA: 3'- cCUGCGC------------GCCCAGGCgu-GCAUCUaUG- -5' |
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2882 | 5' | -56.2 | NC_001493.1 | + | 101596 | 0.66 | 0.882522 |
Target: 5'- aGGACGCGUaGGGUCUGUgacauCGUcuugAGAUcACc -3' miRNA: 3'- -CCUGCGCG-CCCAGGCGu----GCA----UCUA-UG- -5' |
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2882 | 5' | -56.2 | NC_001493.1 | + | 17070 | 0.67 | 0.868137 |
Target: 5'- -cACGCGCGuGGcUCCGUGCGUGcGUGu -3' miRNA: 3'- ccUGCGCGC-CC-AGGCGUGCAUcUAUg -5' |
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2882 | 5' | -56.2 | NC_001493.1 | + | 89209 | 0.67 | 0.868137 |
Target: 5'- uGGA-GCGCGGcGUCCuccccauguacGCGCGUGGugACu -3' miRNA: 3'- -CCUgCGCGCC-CAGG-----------CGUGCAUCuaUG- -5' |
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2882 | 5' | -56.2 | NC_001493.1 | + | 132624 | 0.67 | 0.868137 |
Target: 5'- -cACGCGCGuGGcUCCGUGCGUGcGUGu -3' miRNA: 3'- ccUGCGCGC-CC-AGGCGUGCAUcUAUg -5' |
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2882 | 5' | -56.2 | NC_001493.1 | + | 84713 | 0.67 | 0.860626 |
Target: 5'- uGGGCaG-GCGGGUCUggguggaaGCACuugggGUAGAUGCg -3' miRNA: 3'- -CCUG-CgCGCCCAGG--------CGUG-----CAUCUAUG- -5' |
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2882 | 5' | -56.2 | NC_001493.1 | + | 3809 | 0.67 | 0.858333 |
Target: 5'- uGAUGUGCGGGUCCauccuccuccccggGCAUGacgAGAgcggACg -3' miRNA: 3'- cCUGCGCGCCCAGG--------------CGUGCa--UCUa---UG- -5' |
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2882 | 5' | -56.2 | NC_001493.1 | + | 119363 | 0.67 | 0.858333 |
Target: 5'- uGAUGUGCGGGUCCauccuccuccccggGCAUGacgAGAgcggACg -3' miRNA: 3'- cCUGCGCGCCCAGG--------------CGUGCa--UCUa---UG- -5' |
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2882 | 5' | -56.2 | NC_001493.1 | + | 108774 | 0.67 | 0.844996 |
Target: 5'- cGGACagcgGCGCGGcUCCGauCACGggaggAGGUGCc -3' miRNA: 3'- -CCUG----CGCGCCcAGGC--GUGCa----UCUAUG- -5' |
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2882 | 5' | -56.2 | NC_001493.1 | + | 128396 | 0.67 | 0.83689 |
Target: 5'- cGGGuCGCGUccgGGGUCgGgGCGcucgGGAUACu -3' miRNA: 3'- -CCU-GCGCG---CCCAGgCgUGCa---UCUAUG- -5' |
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2882 | 5' | -56.2 | NC_001493.1 | + | 12842 | 0.67 | 0.83689 |
Target: 5'- cGGGuCGCGUccgGGGUCgGgGCGcucgGGAUACu -3' miRNA: 3'- -CCU-GCGCG---CCCAGgCgUGCa---UCUAUG- -5' |
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2882 | 5' | -56.2 | NC_001493.1 | + | 46638 | 0.67 | 0.828599 |
Target: 5'- aGGACGCuuucgacaccaGCGGGUUCGcCAgGaAGAUAg -3' miRNA: 3'- -CCUGCG-----------CGCCCAGGC-GUgCaUCUAUg -5' |
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2882 | 5' | -56.2 | NC_001493.1 | + | 93309 | 0.68 | 0.82013 |
Target: 5'- uGGAcCGCGaugguuCGGGUgaCCGCGCGgaccGGUGCg -3' miRNA: 3'- -CCU-GCGC------GCCCA--GGCGUGCau--CUAUG- -5' |
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2882 | 5' | -56.2 | NC_001493.1 | + | 114061 | 0.68 | 0.82013 |
Target: 5'- aGAUGCGCGGGgaCUGC-CGUcGGGUAUu -3' miRNA: 3'- cCUGCGCGCCCa-GGCGuGCA-UCUAUG- -5' |
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2882 | 5' | -56.2 | NC_001493.1 | + | 47939 | 0.68 | 0.802692 |
Target: 5'- cGGAgagccCGUGCcccgugaaccucGuGGUCCGCACGcaUAGGUACg -3' miRNA: 3'- -CCU-----GCGCG------------C-CCAGGCGUGC--AUCUAUG- -5' |
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2882 | 5' | -56.2 | NC_001493.1 | + | 125263 | 0.69 | 0.727509 |
Target: 5'- -uACGUGCGGacCCGCGCGUccaucgaggAGAUGCg -3' miRNA: 3'- ccUGCGCGCCcaGGCGUGCA---------UCUAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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