Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2882 | 5' | -56.2 | NC_001493.1 | + | 9709 | 0.69 | 0.727509 |
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Target: 5'- -uACGUGCGGacCCGCGCGUccaucgaggAGAUGCg -3' miRNA: 3'- ccUGCGCGCCcaGGCGUGCA---------UCUAUG- -5' |
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| 2882 | 5' | -56.2 | NC_001493.1 | + | 89526 | 0.7 | 0.687664 |
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Target: 5'- gGGAgCGCGCGGcGUCCGUACcc--GUGCu -3' miRNA: 3'- -CCU-GCGCGCC-CAGGCGUGcaucUAUG- -5' |
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| 2882 | 5' | -56.2 | NC_001493.1 | + | 45880 | 0.71 | 0.647085 |
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Target: 5'- aGAUGUGCGGG-CUGCGCGaGGcgGCc -3' miRNA: 3'- cCUGCGCGCCCaGGCGUGCaUCuaUG- -5' |
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| 2882 | 5' | -56.2 | NC_001493.1 | + | 113327 | 0.73 | 0.516329 |
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Target: 5'- gGGAuCGUGcCGGGUCUGCGCGUcGAg-- -3' miRNA: 3'- -CCU-GCGC-GCCCAGGCGUGCAuCUaug -5' |
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| 2882 | 5' | -56.2 | NC_001493.1 | + | 9863 | 1.1 | 0.002209 |
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Target: 5'- uGGACGCGCGGGUCCGCACGUAGAUACc -3' miRNA: 3'- -CCUGCGCGCCCAGGCGUGCAUCUAUG- -5' |
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| 2882 | 5' | -56.2 | NC_001493.1 | + | 125417 | 1.1 | 0.002209 |
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Target: 5'- uGGACGCGCGGGUCCGCACGUAGAUACc -3' miRNA: 3'- -CCUGCGCGCCCAGGCGUGCAUCUAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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