Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2882 | 5' | -56.2 | NC_001493.1 | + | 101596 | 0.66 | 0.882522 |
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Target: 5'- aGGACGCGUaGGGUCUGUgacauCGUcuugAGAUcACc -3' miRNA: 3'- -CCUGCGCG-CCCAGGCGu----GCA----UCUA-UG- -5' |
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| 2882 | 5' | -56.2 | NC_001493.1 | + | 40836 | 0.66 | 0.882522 |
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Target: 5'- uGACGUGgaugaaaaacccCGGGUCCGagcCGUGGAcGCg -3' miRNA: 3'- cCUGCGC------------GCCCAGGCgu-GCAUCUaUG- -5' |
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| 2882 | 5' | -56.2 | NC_001493.1 | + | 47915 | 0.66 | 0.902435 |
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Target: 5'- cGGACcCGCGGGUCUGgAuCGUcugGGGUuguACa -3' miRNA: 3'- -CCUGcGCGCCCAGGCgU-GCA---UCUA---UG- -5' |
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| 2882 | 5' | -56.2 | NC_001493.1 | + | 48063 | 0.66 | 0.902435 |
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Target: 5'- aGACcCGCGGGUCCGacCGCG-AGcuccAUGCa -3' miRNA: 3'- cCUGcGCGCCCAGGC--GUGCaUC----UAUG- -5' |
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| 2882 | 5' | -56.2 | NC_001493.1 | + | 128524 | 0.66 | 0.902435 |
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Target: 5'- gGGGC-CGgaCGGGUCCGgucacCACGUGGAg-- -3' miRNA: 3'- -CCUGcGC--GCCCAGGC-----GUGCAUCUaug -5' |
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| 2882 | 5' | -56.2 | NC_001493.1 | + | 12970 | 0.66 | 0.902435 |
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Target: 5'- gGGGC-CGgaCGGGUCCGgucacCACGUGGAg-- -3' miRNA: 3'- -CCUGcGC--GCCCAGGC-----GUGCAUCUaug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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