Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2882 | 5' | -56.2 | NC_001493.1 | + | 119363 | 0.67 | 0.858333 |
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Target: 5'- uGAUGUGCGGGUCCauccuccuccccggGCAUGacgAGAgcggACg -3' miRNA: 3'- cCUGCGCGCCCAGG--------------CGUGCa--UCUa---UG- -5' |
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| 2882 | 5' | -56.2 | NC_001493.1 | + | 125263 | 0.69 | 0.727509 |
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Target: 5'- -uACGUGCGGacCCGCGCGUccaucgaggAGAUGCg -3' miRNA: 3'- ccUGCGCGCCcaGGCGUGCA---------UCUAUG- -5' |
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| 2882 | 5' | -56.2 | NC_001493.1 | + | 125417 | 1.1 | 0.002209 |
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Target: 5'- uGGACGCGCGGGUCCGCACGUAGAUACc -3' miRNA: 3'- -CCUGCGCGCCCAGGCGUGCAUCUAUG- -5' |
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| 2882 | 5' | -56.2 | NC_001493.1 | + | 128396 | 0.67 | 0.83689 |
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Target: 5'- cGGGuCGCGUccgGGGUCgGgGCGcucgGGAUACu -3' miRNA: 3'- -CCU-GCGCG---CCCAGgCgUGCa---UCUAUG- -5' |
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| 2882 | 5' | -56.2 | NC_001493.1 | + | 128524 | 0.66 | 0.902435 |
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Target: 5'- gGGGC-CGgaCGGGUCCGgucacCACGUGGAg-- -3' miRNA: 3'- -CCUGcGC--GCCCAGGC-----GUGCAUCUaug -5' |
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| 2882 | 5' | -56.2 | NC_001493.1 | + | 132624 | 0.67 | 0.868137 |
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Target: 5'- -cACGCGCGuGGcUCCGUGCGUGcGUGu -3' miRNA: 3'- ccUGCGCGC-CC-AGGCGUGCAUcUAUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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