Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28836 | 3' | -54.1 | NC_006146.1 | + | 154437 | 0.66 | 0.972929 |
Target: 5'- cCUCcGUCcUCUGgacuaGGGGCCCUGGGCc -3' miRNA: 3'- -GAGaCAGuAGGCg----CUCUGGGACUUGc -5' |
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28836 | 3' | -54.1 | NC_006146.1 | + | 151359 | 0.66 | 0.972929 |
Target: 5'- cCUCcGUCcUCUGgacuaGGGGCCCUGGGCc -3' miRNA: 3'- -GAGaCAGuAGGCg----CUCUGGGACUUGc -5' |
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28836 | 3' | -54.1 | NC_006146.1 | + | 148281 | 0.66 | 0.972929 |
Target: 5'- cCUCcGUCcUCUGgacuaGGGGCCCUGGGCc -3' miRNA: 3'- -GAGaCAGuAGGCg----CUCUGGGACUUGc -5' |
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28836 | 3' | -54.1 | NC_006146.1 | + | 145203 | 0.66 | 0.972929 |
Target: 5'- cCUCcGUCcUCUGgacuaGGGGCCCUGGGCc -3' miRNA: 3'- -GAGaCAGuAGGCg----CUCUGGGACUUGc -5' |
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28836 | 3' | -54.1 | NC_006146.1 | + | 142125 | 0.66 | 0.972929 |
Target: 5'- cCUCcGUCcUCUGgacuaGGGGCCCUGGGCc -3' miRNA: 3'- -GAGaCAGuAGGCg----CUCUGGGACUUGc -5' |
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28836 | 3' | -54.1 | NC_006146.1 | + | 157515 | 0.66 | 0.972929 |
Target: 5'- cCUCcGUCcUCUGgacuaGGGGCCCUGGGCc -3' miRNA: 3'- -GAGaCAGuAGGCg----CUCUGGGACUUGc -5' |
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28836 | 3' | -54.1 | NC_006146.1 | + | 55159 | 0.66 | 0.970116 |
Target: 5'- --aUGUCAgccgCCGgGAGGCCagggUGGACGc -3' miRNA: 3'- gagACAGUa---GGCgCUCUGGg---ACUUGC- -5' |
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28836 | 3' | -54.1 | NC_006146.1 | + | 96374 | 0.66 | 0.960435 |
Target: 5'- uCUUUGUCGggcucugCCGCGGGACCaaGAccACu -3' miRNA: 3'- -GAGACAGUa------GGCGCUCUGGgaCU--UGc -5' |
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28836 | 3' | -54.1 | NC_006146.1 | + | 65642 | 0.67 | 0.956776 |
Target: 5'- aUCUGcUCGUguggcCCuaGAGACCCUGcGACGu -3' miRNA: 3'- gAGAC-AGUA-----GGcgCUCUGGGAC-UUGC- -5' |
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28836 | 3' | -54.1 | NC_006146.1 | + | 160774 | 0.67 | 0.956776 |
Target: 5'- aCUUUGUCGUCCgggggcgaggGUGAGACCCa----- -3' miRNA: 3'- -GAGACAGUAGG----------CGCUCUGGGacuugc -5' |
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28836 | 3' | -54.1 | NC_006146.1 | + | 52304 | 0.67 | 0.956776 |
Target: 5'- --aUGUCAUgCGCGuaggugcuGACCCUGA-CGu -3' miRNA: 3'- gagACAGUAgGCGCu-------CUGGGACUuGC- -5' |
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28836 | 3' | -54.1 | NC_006146.1 | + | 41039 | 0.67 | 0.952893 |
Target: 5'- uCUCcccccCGUCCGCGGcGGCCCUGGGgGc -3' miRNA: 3'- -GAGaca--GUAGGCGCU-CUGGGACUUgC- -5' |
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28836 | 3' | -54.1 | NC_006146.1 | + | 86295 | 0.67 | 0.948357 |
Target: 5'- cCUCUGUC--CgGCauggcuggaguagGAGGCCCUGGGCa -3' miRNA: 3'- -GAGACAGuaGgCG-------------CUCUGGGACUUGc -5' |
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28836 | 3' | -54.1 | NC_006146.1 | + | 68782 | 0.67 | 0.93939 |
Target: 5'- gCUCUGUCAUCUGgaacaccaUGAucucucuGGCCgUGAGCGg -3' miRNA: 3'- -GAGACAGUAGGC--------GCU-------CUGGgACUUGC- -5' |
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28836 | 3' | -54.1 | NC_006146.1 | + | 15451 | 0.68 | 0.929997 |
Target: 5'- -cCUGgcgCAgCagaGCGAGACCCUGAcCGa -3' miRNA: 3'- gaGACa--GUaGg--CGCUCUGGGACUuGC- -5' |
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28836 | 3' | -54.1 | NC_006146.1 | + | 50301 | 0.68 | 0.924708 |
Target: 5'- aUCUGUCAggcgCCGCcGGGCCUUGucCa -3' miRNA: 3'- gAGACAGUa---GGCGcUCUGGGACuuGc -5' |
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28836 | 3' | -54.1 | NC_006146.1 | + | 111622 | 0.68 | 0.912827 |
Target: 5'- -aCUGcCAgcccaggUCCGCGAagacgucggccaGGCCCUGAACa -3' miRNA: 3'- gaGACaGU-------AGGCGCU------------CUGGGACUUGc -5' |
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28836 | 3' | -54.1 | NC_006146.1 | + | 42130 | 0.68 | 0.907416 |
Target: 5'- cCUCUGgCAcgCCGUGu--CCCUGAGCGu -3' miRNA: 3'- -GAGACaGUa-GGCGCucuGGGACUUGC- -5' |
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28836 | 3' | -54.1 | NC_006146.1 | + | 161759 | 0.69 | 0.894714 |
Target: 5'- -gCUGagGUCCGagGGGGCgCCUGGGCGg -3' miRNA: 3'- gaGACagUAGGCg-CUCUG-GGACUUGC- -5' |
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28836 | 3' | -54.1 | NC_006146.1 | + | 14532 | 0.69 | 0.892063 |
Target: 5'- uUUUGUC-UCCGCGugcgcauaauggcGGCCCUGGACc -3' miRNA: 3'- gAGACAGuAGGCGCu------------CUGGGACUUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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