Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28847 | 5' | -56.1 | NC_006146.1 | + | 46219 | 0.7 | 0.65341 |
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Target: 5'- gUUUCAGGACGaucucccugGGUGCUGGAcgGAGAUu -3' miRNA: 3'- -AAGGUCCUGUa--------CCACGGCCUa-CUCUA- -5' |
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| 28847 | 5' | -56.1 | NC_006146.1 | + | 63829 | 0.71 | 0.643041 |
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Target: 5'- aUCCAGGucuugcCGUGGcGCCGGGgaaUGAGAa -3' miRNA: 3'- aAGGUCCu-----GUACCaCGGCCU---ACUCUa -5' |
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| 28847 | 5' | -56.1 | NC_006146.1 | + | 90049 | 0.72 | 0.54017 |
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Target: 5'- --gCAGGAgGUGGUGCUGGA-GGGAg -3' miRNA: 3'- aagGUCCUgUACCACGGCCUaCUCUa -5' |
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| 28847 | 5' | -56.1 | NC_006146.1 | + | 124307 | 0.72 | 0.539162 |
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Target: 5'- cUUCCAGGGCcaggccaAUGGUGCUGGGUGucuuguAGAUu -3' miRNA: 3'- -AAGGUCCUG-------UACCACGGCCUAC------UCUA- -5' |
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| 28847 | 5' | -56.1 | NC_006146.1 | + | 71475 | 0.73 | 0.487777 |
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Target: 5'- uUUCCAGGGCcugccguuuggccgGUGGUGCCuGGggGAGGc -3' miRNA: 3'- -AAGGUCCUG--------------UACCACGG-CCuaCUCUa -5' |
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| 28847 | 5' | -56.1 | NC_006146.1 | + | 56815 | 1.03 | 0.006579 |
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Target: 5'- cUUCCAGGACAUGGUGCCGGAUGAGAUu -3' miRNA: 3'- -AAGGUCCUGUACCACGGCCUACUCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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