Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28847 | 5' | -56.1 | NC_006146.1 | + | 56815 | 1.03 | 0.006579 |
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Target: 5'- cUUCCAGGACAUGGUGCCGGAUGAGAUu -3' miRNA: 3'- -AAGGUCCUGUACCACGGCCUACUCUA- -5' |
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| 28847 | 5' | -56.1 | NC_006146.1 | + | 51210 | 0.69 | 0.744853 |
|
Target: 5'- --aCAGGGCAUccacgaggccggGGUGgCGGGUGAGGUu -3' miRNA: 3'- aagGUCCUGUA------------CCACgGCCUACUCUA- -5' |
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| 28847 | 5' | -56.1 | NC_006146.1 | + | 50851 | 0.67 | 0.836533 |
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Target: 5'- gUCCAGG-C-UGGUGggGGAUGAGAg -3' miRNA: 3'- aAGGUCCuGuACCACggCCUACUCUa -5' |
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| 28847 | 5' | -56.1 | NC_006146.1 | + | 46219 | 0.7 | 0.65341 |
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Target: 5'- gUUUCAGGACGaucucccugGGUGCUGGAcgGAGAUu -3' miRNA: 3'- -AAGGUCCUGUa--------CCACGGCCUa-CUCUA- -5' |
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| 28847 | 5' | -56.1 | NC_006146.1 | + | 33345 | 0.67 | 0.852847 |
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Target: 5'- cUCCGGGGCAgccgGGUGgCCGccGGUGGGu- -3' miRNA: 3'- aAGGUCCUGUa---CCAC-GGC--CUACUCua -5' |
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| 28847 | 5' | -56.1 | NC_006146.1 | + | 2845 | 0.67 | 0.84479 |
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Target: 5'- -gCCAGGAgCggGGUGCCGGuUGuGGc -3' miRNA: 3'- aaGGUCCU-GuaCCACGGCCuACuCUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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