Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28876 | 5' | -53.4 | NC_006146.1 | + | 70541 | 0.68 | 0.907274 |
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Target: 5'- gUCg-GAGGGCGGugguggGGUAUAUGGGUUGu -3' miRNA: 3'- aAGgaUUCCCGCC------UCGUGUACCUAAU- -5' |
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| 28876 | 5' | -53.4 | NC_006146.1 | + | 66669 | 0.68 | 0.894032 |
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Target: 5'- aUCCUGaggGGGGCGGGGCACc------- -3' miRNA: 3'- aAGGAU---UCCCGCCUCGUGuaccuaau -5' |
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| 28876 | 5' | -53.4 | NC_006146.1 | + | 162040 | 0.68 | 0.887039 |
|
Target: 5'- -gCCUGGGGGCgGGGGCAgGcuguuuaaccUGGAUg- -3' miRNA: 3'- aaGGAUUCCCG-CCUCGUgU----------ACCUAau -5' |
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| 28876 | 5' | -53.4 | NC_006146.1 | + | 108160 | 0.69 | 0.856695 |
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Target: 5'- -cCCUGAGGGCaaagaaGAGCACAgcacUGGAg-- -3' miRNA: 3'- aaGGAUUCCCGc-----CUCGUGU----ACCUaau -5' |
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| 28876 | 5' | -53.4 | NC_006146.1 | + | 135725 | 0.85 | 0.141831 |
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Target: 5'- gUUUCUAAaGGCGGGGCACAUGGAUUAg -3' miRNA: 3'- -AAGGAUUcCCGCCUCGUGUACCUAAU- -5' |
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| 28876 | 5' | -53.4 | NC_006146.1 | + | 31893 | 1.05 | 0.007778 |
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Target: 5'- uUUCCUAAGGGCGGAGCACAUGGAUUAg -3' miRNA: 3'- -AAGGAUUCCCGCCUCGUGUACCUAAU- -5' |
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| 28876 | 5' | -53.4 | NC_006146.1 | + | 89582 | 0.66 | 0.958183 |
|
Target: 5'- -----uGGGGCGGAGgACuUGGGUUGc -3' miRNA: 3'- aaggauUCCCGCCUCgUGuACCUAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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