Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2889 | 3' | -61.1 | NC_001493.1 | + | 47926 | 0.66 | 0.690608 |
Target: 5'- cCCCGuGaACCUCGUGGUCCgCaCGCAUa -3' miRNA: 3'- aGGGC-C-UGGAGUGCCAGGaGcGCGUG- -5' |
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2889 | 3' | -61.1 | NC_001493.1 | + | 131446 | 0.66 | 0.690608 |
Target: 5'- aCCgGGAUCagCGCGGUgaUCGCGgACa -3' miRNA: 3'- aGGgCCUGGa-GUGCCAggAGCGCgUG- -5' |
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2889 | 3' | -61.1 | NC_001493.1 | + | 15892 | 0.66 | 0.690608 |
Target: 5'- aCCgGGAUCagCGCGGUgaUCGCGgACa -3' miRNA: 3'- aGGgCCUGGa-GUGCCAggAGCGCgUG- -5' |
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2889 | 3' | -61.1 | NC_001493.1 | + | 5449 | 0.66 | 0.641261 |
Target: 5'- aUCCCacuGGAUCUCACaGaUCCUCGC-CAg -3' miRNA: 3'- -AGGG---CCUGGAGUGcC-AGGAGCGcGUg -5' |
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2889 | 3' | -61.1 | NC_001493.1 | + | 121003 | 0.66 | 0.641261 |
Target: 5'- aUCCCacuGGAUCUCACaGaUCCUCGC-CAg -3' miRNA: 3'- -AGGG---CCUGGAGUGcC-AGGAGCGcGUg -5' |
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2889 | 3' | -61.1 | NC_001493.1 | + | 109334 | 0.67 | 0.631341 |
Target: 5'- cUCCCGGGCUcCAUa--CCaUCGCGCACc -3' miRNA: 3'- -AGGGCCUGGaGUGccaGG-AGCGCGUG- -5' |
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2889 | 3' | -61.1 | NC_001493.1 | + | 22408 | 0.67 | 0.621422 |
Target: 5'- cUUCCGGACCUaccgGCGG-CC-CGCGUg- -3' miRNA: 3'- -AGGGCCUGGAg---UGCCaGGaGCGCGug -5' |
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2889 | 3' | -61.1 | NC_001493.1 | + | 8414 | 0.67 | 0.621422 |
Target: 5'- aCCCGGACCUUcggcuGCGGcuguggaCC-CGgGCACc -3' miRNA: 3'- aGGGCCUGGAG-----UGCCa------GGaGCgCGUG- -5' |
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2889 | 3' | -61.1 | NC_001493.1 | + | 123968 | 0.67 | 0.621422 |
Target: 5'- aCCCGGACCUUcggcuGCGGcuguggaCC-CGgGCACc -3' miRNA: 3'- aGGGCCUGGAG-----UGCCa------GGaGCgCGUG- -5' |
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2889 | 3' | -61.1 | NC_001493.1 | + | 13716 | 0.67 | 0.61151 |
Target: 5'- gUCUCGGGCCUCuccaccGCGGUCCg-GgGUAa -3' miRNA: 3'- -AGGGCCUGGAG------UGCCAGGagCgCGUg -5' |
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2889 | 3' | -61.1 | NC_001493.1 | + | 40052 | 0.67 | 0.61151 |
Target: 5'- aCUCGGaACCcgaGCgGGUCCU-GCGCACg -3' miRNA: 3'- aGGGCC-UGGag-UG-CCAGGAgCGCGUG- -5' |
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2889 | 3' | -61.1 | NC_001493.1 | + | 37711 | 0.67 | 0.605569 |
Target: 5'- cCCCGGGCgaCcCGGucgacgaccccuccaUCCcCGCGCACa -3' miRNA: 3'- aGGGCCUGgaGuGCC---------------AGGaGCGCGUG- -5' |
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2889 | 3' | -61.1 | NC_001493.1 | + | 129270 | 0.67 | 0.601613 |
Target: 5'- gUCUCGGGCCUCuccaccGCGGUCCgggguaaggGgGUACg -3' miRNA: 3'- -AGGGCCUGGAG------UGCCAGGag-------CgCGUG- -5' |
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2889 | 3' | -61.1 | NC_001493.1 | + | 20852 | 0.67 | 0.601613 |
Target: 5'- aCCUGGGCCUCGuuGUUaaaaaCGUGCACg -3' miRNA: 3'- aGGGCCUGGAGUgcCAGga---GCGCGUG- -5' |
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2889 | 3' | -61.1 | NC_001493.1 | + | 80978 | 0.67 | 0.600624 |
Target: 5'- uUCCUGGGggugaauCCUCGgGGUCCgcaacaGCGCGg -3' miRNA: 3'- -AGGGCCU-------GGAGUgCCAGGag----CGCGUg -5' |
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2889 | 3' | -61.1 | NC_001493.1 | + | 36541 | 0.67 | 0.591737 |
Target: 5'- cCCCGaGCCUaagacCGCGGUCgaggccaUCGUGCGCg -3' miRNA: 3'- aGGGCcUGGA-----GUGCCAGg------AGCGCGUG- -5' |
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2889 | 3' | -61.1 | NC_001493.1 | + | 101470 | 0.67 | 0.57894 |
Target: 5'- aCCCGGaguGCCUcCACuGccuuuuugaugacuUCCUUGCGCGCa -3' miRNA: 3'- aGGGCC---UGGA-GUGcC--------------AGGAGCGCGUG- -5' |
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2889 | 3' | -61.1 | NC_001493.1 | + | 118604 | 0.68 | 0.572074 |
Target: 5'- aUCCUcGACCUCGuccgGGUCCUCGUcCGCc -3' miRNA: 3'- -AGGGcCUGGAGUg---CCAGGAGCGcGUG- -5' |
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2889 | 3' | -61.1 | NC_001493.1 | + | 3049 | 0.68 | 0.572074 |
Target: 5'- aUCCUcGACCUCGuccgGGUCCUCGUcCGCc -3' miRNA: 3'- -AGGGcCUGGAGUg---CCAGGAGCGcGUG- -5' |
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2889 | 3' | -61.1 | NC_001493.1 | + | 18923 | 0.68 | 0.571095 |
Target: 5'- cUCCCGGGgguugguCCUCAgGcaCC-CGCGCGCg -3' miRNA: 3'- -AGGGCCU-------GGAGUgCcaGGaGCGCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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