Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2889 | 3' | -61.1 | NC_001493.1 | + | 13716 | 0.67 | 0.61151 |
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Target: 5'- gUCUCGGGCCUCuccaccGCGGUCCg-GgGUAa -3' miRNA: 3'- -AGGGCCUGGAG------UGCCAGGagCgCGUg -5' |
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| 2889 | 3' | -61.1 | NC_001493.1 | + | 8414 | 0.67 | 0.621422 |
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Target: 5'- aCCCGGACCUUcggcuGCGGcuguggaCC-CGgGCACc -3' miRNA: 3'- aGGGCCUGGAG-----UGCCa------GGaGCgCGUG- -5' |
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| 2889 | 3' | -61.1 | NC_001493.1 | + | 22408 | 0.67 | 0.621422 |
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Target: 5'- cUUCCGGACCUaccgGCGG-CC-CGCGUg- -3' miRNA: 3'- -AGGGCCUGGAg---UGCCaGGaGCGCGug -5' |
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| 2889 | 3' | -61.1 | NC_001493.1 | + | 123968 | 0.67 | 0.621422 |
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Target: 5'- aCCCGGACCUUcggcuGCGGcuguggaCC-CGgGCACc -3' miRNA: 3'- aGGGCCUGGAG-----UGCCa------GGaGCgCGUG- -5' |
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| 2889 | 3' | -61.1 | NC_001493.1 | + | 109334 | 0.67 | 0.631341 |
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Target: 5'- cUCCCGGGCUcCAUa--CCaUCGCGCACc -3' miRNA: 3'- -AGGGCCUGGaGUGccaGG-AGCGCGUG- -5' |
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| 2889 | 3' | -61.1 | NC_001493.1 | + | 121003 | 0.66 | 0.641261 |
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Target: 5'- aUCCCacuGGAUCUCACaGaUCCUCGC-CAg -3' miRNA: 3'- -AGGG---CCUGGAGUGcC-AGGAGCGcGUg -5' |
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| 2889 | 3' | -61.1 | NC_001493.1 | + | 5449 | 0.66 | 0.641261 |
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Target: 5'- aUCCCacuGGAUCUCACaGaUCCUCGC-CAg -3' miRNA: 3'- -AGGG---CCUGGAGUGcC-AGGAGCGcGUg -5' |
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| 2889 | 3' | -61.1 | NC_001493.1 | + | 15892 | 0.66 | 0.690608 |
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Target: 5'- aCCgGGAUCagCGCGGUgaUCGCGgACa -3' miRNA: 3'- aGGgCCUGGa-GUGCCAggAGCGCgUG- -5' |
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| 2889 | 3' | -61.1 | NC_001493.1 | + | 131446 | 0.66 | 0.690608 |
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Target: 5'- aCCgGGAUCagCGCGGUgaUCGCGgACa -3' miRNA: 3'- aGGgCCUGGa-GUGCCAggAGCGCgUG- -5' |
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| 2889 | 3' | -61.1 | NC_001493.1 | + | 47926 | 0.66 | 0.690608 |
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Target: 5'- cCCCGuGaACCUCGUGGUCCgCaCGCAUa -3' miRNA: 3'- aGGGC-C-UGGAGUGCCAGGaGcGCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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