Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2889 | 3' | -61.1 | NC_001493.1 | + | 80978 | 0.67 | 0.600624 |
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Target: 5'- uUCCUGGGggugaauCCUCGgGGUCCgcaacaGCGCGg -3' miRNA: 3'- -AGGGCCU-------GGAGUgCCAGGag----CGCGUg -5' |
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| 2889 | 3' | -61.1 | NC_001493.1 | + | 36541 | 0.67 | 0.591737 |
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Target: 5'- cCCCGaGCCUaagacCGCGGUCgaggccaUCGUGCGCg -3' miRNA: 3'- aGGGCcUGGA-----GUGCCAGg------AGCGCGUG- -5' |
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| 2889 | 3' | -61.1 | NC_001493.1 | + | 101470 | 0.67 | 0.57894 |
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Target: 5'- aCCCGGaguGCCUcCACuGccuuuuugaugacuUCCUUGCGCGCa -3' miRNA: 3'- aGGGCC---UGGA-GUGcC--------------AGGAGCGCGUG- -5' |
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| 2889 | 3' | -61.1 | NC_001493.1 | + | 17995 | 0.69 | 0.49545 |
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Target: 5'- aUCCuCGccGACCUCACGGggaagcUCCUgGCGCuCa -3' miRNA: 3'- -AGG-GC--CUGGAGUGCC------AGGAgCGCGuG- -5' |
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| 2889 | 3' | -61.1 | NC_001493.1 | + | 133549 | 0.69 | 0.49545 |
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Target: 5'- aUCCuCGccGACCUCACGGggaagcUCCUgGCGCuCa -3' miRNA: 3'- -AGG-GC--CUGGAGUGCC------AGGAgCGCGuG- -5' |
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| 2889 | 3' | -61.1 | NC_001493.1 | + | 110735 | 0.7 | 0.458891 |
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Target: 5'- -gCgGGACCUCGuCGGUacCCUCGCgGCAa -3' miRNA: 3'- agGgCCUGGAGU-GCCA--GGAGCG-CGUg -5' |
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| 2889 | 3' | -61.1 | NC_001493.1 | + | 93036 | 0.72 | 0.321787 |
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Target: 5'- gCCCGGAgCUCACGGUCg-UGUGuCACc -3' miRNA: 3'- aGGGCCUgGAGUGCCAGgaGCGC-GUG- -5' |
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| 2889 | 3' | -61.1 | NC_001493.1 | + | 14592 | 1.1 | 0.000829 |
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Target: 5'- uUCCCGGACCUCACGGUCCUCGCGCACg -3' miRNA: 3'- -AGGGCCUGGAGUGCCAGGAGCGCGUG- -5' |
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| 2889 | 3' | -61.1 | NC_001493.1 | + | 130147 | 1.1 | 0.000829 |
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Target: 5'- uUCCCGGACCUCACGGUCCUCGCGCACg -3' miRNA: 3'- -AGGGCCUGGAGUGCCAGGAGCGCGUG- -5' |
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| 2889 | 3' | -61.1 | NC_001493.1 | + | 47926 | 0.66 | 0.690608 |
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Target: 5'- cCCCGuGaACCUCGUGGUCCgCaCGCAUa -3' miRNA: 3'- aGGGC-C-UGGAGUGCCAGGaGcGCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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