Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28996 | 5' | -52.4 | NC_006146.1 | + | 127139 | 0.66 | 0.981514 |
Target: 5'- gUCCCc-UCUGCGGCCCacuccucUCUGUc- -3' miRNA: 3'- -AGGGauAGACGUCGGGaaa----AGACGuc -5' |
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28996 | 5' | -52.4 | NC_006146.1 | + | 159651 | 0.66 | 0.9793 |
Target: 5'- cUCCUUGUCguacaGCGGgaCCCUaaagCUGCAGa -3' miRNA: 3'- -AGGGAUAGa----CGUC--GGGAaaa-GACGUC- -5' |
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28996 | 5' | -52.4 | NC_006146.1 | + | 15434 | 0.66 | 0.976895 |
Target: 5'- gCCCgg-CUGCAGUCCUgccuggCgcaGCAGa -3' miRNA: 3'- aGGGauaGACGUCGGGAaaa---Ga--CGUC- -5' |
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28996 | 5' | -52.4 | NC_006146.1 | + | 121886 | 0.66 | 0.970891 |
Target: 5'- cCCCggagaacgccCUGCGGCuCCUagccggUCUGCAGa -3' miRNA: 3'- aGGGaua-------GACGUCG-GGAaa----AGACGUC- -5' |
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28996 | 5' | -52.4 | NC_006146.1 | + | 123617 | 0.67 | 0.968453 |
Target: 5'- gUCCCgg-CUGCAGgacg-UUCUGCAGg -3' miRNA: 3'- -AGGGauaGACGUCgggaaAAGACGUC- -5' |
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28996 | 5' | -52.4 | NC_006146.1 | + | 127778 | 0.67 | 0.962089 |
Target: 5'- gCCCUG-CUGCAGCUCUUgaaauuuaggaaGCAGg -3' miRNA: 3'- aGGGAUaGACGUCGGGAAaaga--------CGUC- -5' |
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28996 | 5' | -52.4 | NC_006146.1 | + | 42467 | 0.67 | 0.960643 |
Target: 5'- cUCCCgcccgGUCUGCGGCCCggaucacccuccccUggccgugUUCUGCc- -3' miRNA: 3'- -AGGGa----UAGACGUCGGG--------------Aa------AAGACGuc -5' |
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28996 | 5' | -52.4 | NC_006146.1 | + | 26694 | 0.67 | 0.957639 |
Target: 5'- gCCCUGUCUagggccgggagagGCAGCCCcgag--GCGGg -3' miRNA: 3'- aGGGAUAGA-------------CGUCGGGaaaagaCGUC- -5' |
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28996 | 5' | -52.4 | NC_006146.1 | + | 23616 | 0.67 | 0.957639 |
Target: 5'- gCCCUGUCUagggccgggagagGCAGCCCcgag--GCGGg -3' miRNA: 3'- aGGGAUAGA-------------CGUCGGGaaaagaCGUC- -5' |
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28996 | 5' | -52.4 | NC_006146.1 | + | 20538 | 0.67 | 0.957639 |
Target: 5'- gCCCUGUCUagggccgggagagGCAGCCCcgag--GCGGg -3' miRNA: 3'- aGGGAUAGA-------------CGUCGGGaaaagaCGUC- -5' |
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28996 | 5' | -52.4 | NC_006146.1 | + | 17460 | 0.67 | 0.957639 |
Target: 5'- gCCCUGUCUagggccgggagagGCAGCCCcgag--GCGGg -3' miRNA: 3'- aGGGAUAGA-------------CGUCGGGaaaagaCGUC- -5' |
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28996 | 5' | -52.4 | NC_006146.1 | + | 14382 | 0.67 | 0.957639 |
Target: 5'- gCCCUGUCUagggccgggagagGCAGCCCcgag--GCGGg -3' miRNA: 3'- aGGGAUAGA-------------CGUCGGGaaaagaCGUC- -5' |
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28996 | 5' | -52.4 | NC_006146.1 | + | 62928 | 0.67 | 0.954075 |
Target: 5'- gCCCUGUCUGUggucuccagGGCCCUggaggUCU-CGGc -3' miRNA: 3'- aGGGAUAGACG---------UCGGGAaa---AGAcGUC- -5' |
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28996 | 5' | -52.4 | NC_006146.1 | + | 1689 | 0.68 | 0.949884 |
Target: 5'- aUCCUUAaguUCUGCAgGCUCUgcugUUUCUGCu- -3' miRNA: 3'- -AGGGAU---AGACGU-CGGGA----AAAGACGuc -5' |
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28996 | 5' | -52.4 | NC_006146.1 | + | 161334 | 0.69 | 0.900824 |
Target: 5'- aCCCUGUCUGUucaGGCCaagaggUCUGUAu -3' miRNA: 3'- aGGGAUAGACG---UCGGgaaa--AGACGUc -5' |
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28996 | 5' | -52.4 | NC_006146.1 | + | 131120 | 0.7 | 0.89411 |
Target: 5'- gUCCCUAUCUccaGcCAGCaCCUgccgcgUCUGCGa -3' miRNA: 3'- -AGGGAUAGA---C-GUCG-GGAaa----AGACGUc -5' |
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28996 | 5' | -52.4 | NC_006146.1 | + | 8563 | 0.7 | 0.856964 |
Target: 5'- gCUCauagCUGCAGCCCggg-CUGCAGc -3' miRNA: 3'- aGGGaua-GACGUCGGGaaaaGACGUC- -5' |
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28996 | 5' | -52.4 | NC_006146.1 | + | 85277 | 0.72 | 0.808989 |
Target: 5'- cCCCUGUCUGCcucucuGGCCCUUugccccggugccagcUUCcgagGCGGg -3' miRNA: 3'- aGGGAUAGACG------UCGGGAA---------------AAGa---CGUC- -5' |
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28996 | 5' | -52.4 | NC_006146.1 | + | 101933 | 0.73 | 0.747706 |
Target: 5'- cUCCCUAUCaGCGGCUCUgccCUGCc- -3' miRNA: 3'- -AGGGAUAGaCGUCGGGAaaaGACGuc -5' |
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28996 | 5' | -52.4 | NC_006146.1 | + | 60857 | 0.74 | 0.686176 |
Target: 5'- uUCCCUGUUauacaauagcaUGCAGCCC----CUGCAGa -3' miRNA: 3'- -AGGGAUAG-----------ACGUCGGGaaaaGACGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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