Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29135 | 5' | -57.6 | NC_006146.1 | + | 26498 | 0.67 | 0.832346 |
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Target: 5'- uGCCCUCUgagcccCCUUUGCCCccUGGcagGCCa -3' miRNA: 3'- uCGGGAGA------GGGAACGGGcaACU---UGGc -5' |
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| 29135 | 5' | -57.6 | NC_006146.1 | + | 73033 | 0.67 | 0.840449 |
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Target: 5'- aGGCCCUCgUUCCacgGCCCG--GggUCGg -3' miRNA: 3'- -UCGGGAG-AGGGaa-CGGGCaaCuuGGC- -5' |
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| 29135 | 5' | -57.6 | NC_006146.1 | + | 74255 | 0.67 | 0.840449 |
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Target: 5'- cGGCCgUCUCCCcgggcugGCCaaa-GAGCCGg -3' miRNA: 3'- -UCGGgAGAGGGaa-----CGGgcaaCUUGGC- -5' |
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| 29135 | 5' | -57.6 | NC_006146.1 | + | 5185 | 0.66 | 0.847585 |
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Target: 5'- aGGCCCgagacguUUUCCCUggGCCUGgggGAGCUa -3' miRNA: 3'- -UCGGG-------AGAGGGAa-CGGGCaa-CUUGGc -5' |
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| 29135 | 5' | -57.6 | NC_006146.1 | + | 2759 | 0.66 | 0.848368 |
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Target: 5'- cGGCCCUCgcaggCCCaaGCCCGgaugucaggGAagacGCCa -3' miRNA: 3'- -UCGGGAGa----GGGaaCGGGCaa-------CU----UGGc -5' |
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| 29135 | 5' | -57.6 | NC_006146.1 | + | 125777 | 0.66 | 0.856098 |
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Target: 5'- aAGCCCaaggCUCCCggcgGUcaCCGgccGAGCCGg -3' miRNA: 3'- -UCGGGa---GAGGGaa--CG--GGCaa-CUUGGC- -5' |
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| 29135 | 5' | -57.6 | NC_006146.1 | + | 149502 | 0.66 | 0.870964 |
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Target: 5'- uGCCCUCUCugagguCCgcauagGCCuCGUUGAggaugucgaugACCGg -3' miRNA: 3'- uCGGGAGAG------GGaa----CGG-GCAACU-----------UGGC- -5' |
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| 29135 | 5' | -57.6 | NC_006146.1 | + | 15809 | 0.66 | 0.878089 |
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Target: 5'- -uUCCUgacCUCCCUgaucugGCCCGgcaucGAGCCGa -3' miRNA: 3'- ucGGGA---GAGGGAa-----CGGGCaa---CUUGGC- -5' |
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| 29135 | 5' | -57.6 | NC_006146.1 | + | 33523 | 0.66 | 0.878089 |
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Target: 5'- cGGCCUccCUCCCUagguugUGCCCGgcuuGCCu -3' miRNA: 3'- -UCGGGa-GAGGGA------ACGGGCaacuUGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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